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H7039

Sigma-Aldrich

3-Hydroxy-3-methylglutaryl-CoA reductase human

solution

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Synonym(s):
HMGCoA Reductase, HMGR
Enzyme Commission number:
NACRES:
NA.54

assay

≥90% (SDS-PAGE)

Quality Level

form

solution

specific activity

2-8 units/mg protein

mol wt

~76 kDa (for the fusion protein)

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... HMGCR(3156)

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This Item
SAB4200528SAB4200529ABS229
vibrant-m

ABS229

Anti-HMG-CoA reductase Antibody

assay

≥90% (SDS-PAGE)

assay

-

assay

-

assay

-

specific activity

2-8 units/mg protein

specific activity

-

specific activity

-

specific activity

-

form

solution

form

buffered aqueous solution

form

buffered aqueous solution

form

-

storage temp.

−70°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

Application

3-Hydroxy-3-methylglutaryl-CoA reductase human has been used:
  • in anti-HMGCR enzyme linked immunosorbent assay (ELISA)
  • as an antigen to measure anti-HMGCR antibodies by blot analysis
  • in HMG-CoA reductase activity assays
  • as an antigen to compare in-house antigen via sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE)/western blot

Biochem/physiol Actions

3-Hydroxy-3-methylglutaryl-CoA reductase/HMGCR serves as a rate limiting enzyme in mevalonate pathway. It also controls the oncogenic Hippo transducers TAZ/YAP. HMGCR also perform as a genetic modifier for Alzheimer′s disease (AD).
Hydroxy-3-methylglutaryl-CoA reductase catalyzes the committed step in cholesterol biosynthesis.

Physical properties

Consists of the HMG CoA reductase catalytic domain fused to GST protein.

Unit Definition

One unit will convert 1.0 μmole of NADPH to NADP+ per minute at 37 °C.

Physical form

Supplied as a solution containing 250 μg protein in 50 mM Tris pH 7.5, with 5 mM DTT, 1:200 Protease Inhibitor Cocktail (Catalog Number P8340) and 50% (w/v) glycerol.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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25G
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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

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Myopathy with anti-HMGCR antibodies Perimysium and myofiber pathology
Alshehri A, et al.
Neurology? neuroimmunology & neuroinflammation, 2(4), e124-e124 (2015)
OP0009 Distinctive Immunofluorescence Pattern in Statin-Associated Autoimmune Myopathy with Anti-HMGCR Autoantibodies
Alvarado-Cardenas M, et al.
Annals of the Rheumatic Diseases, 75, 56-56 (2016)
Analysis of autoantibodies to 3-hydroxy-3-methylglutaryl-coenzyme A reductase using different technologies
Musset L, et al.
Journal of immunology research, 2014 (2014)
ChemVassa: a new method for identifying small molecule hits in drug discovery
Moldover B, et al.
The Open Medicinal Chemistry Journal, 6, 29-29 (2012)
HMG-CoAR expression in male breast cancer: relationship with hormone receptors, Hippo transducers and survival outcomes
Di BA, et al.
Scientific reports, 6, 35121-35121 (2016)

Articles

Biosynthesis of cholesterol generally takes place in the endoplasmic reticulum of hepatic cells and begins with acetyl- CoA, which is mainly derived from an oxidation reaction in the mitochondria. Acetyl-CoA and acetoacetyl-CoA are converted to 3-hydroxy- 3-methylglutaryl-CoA (HMG-CoA) by HMG-CoA synthase.

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