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I8015

Sigma-Aldrich

IgG from rat serum

technical grade, ≥80% (SDS-PAGE), buffered aqueous solution

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Synonym(s):
Rat IgG
MDL number:
NACRES:
NA.46

conjugate

unconjugated

grade

technical grade

assay

≥80% (SDS-PAGE)

form

buffered aqueous solution

shipped in

dry ice

storage temp.

−20°C

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This Item
I9140I2511M1421
vibrant-m

I8015

IgG from rat serum

vibrant-m

I9140

IgG from goat serum

vibrant-m

I2511

IgG from human serum

vibrant-m

M1421

IgA, Kappa from murine myeloma

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

grade

technical grade

grade

technical grade

grade

reagent grade

grade

-

assay

≥80% (SDS-PAGE)

assay

≥80% (SDS-PAGE)

assay

≥95% (HPLC)

assay

≥90% (non-reduced, SDS-PAGE)

General description

IgG (immunoglobulin G) antibody is enriched in mother′s milk. IgG is a major class of immunoglobulin. It contributes to 10−20% of plasma protein and is regarded as one of the most abundant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50kDa and 25kDa respectively. The chains are interlinked with a disulfide bond. Both the L and the H chains consist of a variable domain at the N-terminal. The C-terminal bears′ constant domains: three in H chain with a hinge region and one in the L chain. The variable region of IgG antibody is specific to antigens and is highly conserved.

Application

IgG from rat serum has been used:
  • To block programmed death ligand 1.
  • As a control antibody to study the role of integrin b1 in spindle morphology
  • In FACS (flow cytometry) staining
  • ELISA (enzyme linked immunosorbent assay)

IgG from rat serum was used to block non-specific staining during FACS of peritoneal cells isolated from BALB/c mice. It was used as positive control in VDR staining of male and female breast tumor cells. IgG from rat serum was injected in control mice in tumor transplantation studies.

Biochem/physiol Actions

IgG antibody has its function similar to IgM antibody in complement system activation. IgG participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody-dependent cell-mediated cytotoxicity.
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.2, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

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Irradiation-tolerant lung cancer cells acquire invasive ability dependent on dephosphorylation of the myosin regulatory light chain
Ishihara S, et al.
Febs Letters, 587(6), 732-736 (2013)
M Boesen et al.
Clinical and experimental immunology, 121(2), 210-215 (2000-08-10)
An attempt was made to identify the selection pressures put upon a growing tumour by CD8+ T cells. To this end tumours induced with 3-methylcholanthrene in T cell-deficient nude mice and in congenic T cell-competent nu/+ mice were transplanted to
The Immunoglobulins: Structure and Function (1998)
M J Janatpour et al.
The Journal of experimental medicine, 194(9), 1375-1384 (2001-11-07)
Monocytes recruited from the blood are key contributors to the nature of an immune response. While monocyte recruitment in a subset of immunopathologies has been well studied and largely attributed to the chemokine monocyte chemoattractant protein (MCP)-1, mechanisms mediating such
The Laboratory Rat (2005)

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