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I8779

Sigma-Aldrich

Insulin-like Growth Factor-I from mouse

IGF-I, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

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Synonym(s):
IGF-I
MDL number:
NACRES:
NA.77

biological source

mouse

Quality Level

recombinant

expressed in E. coli

Assay

≥98% (SDS-PAGE and HPLC)

form

lyophilized powder

quality

endotoxin tested

mol wt

predicted mol wt ~7.6 kDa

packaging

glass bottle of 50 μg (in glass insert)

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 ng/mg (LAL test)

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... IGF1(3479)
mouse ... Igf1(16000)

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This Item
SRP3069I2526I3769
Sigma-Aldrich

Sigma-Aldrich

SRP3069

IGF-I human

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

≥98% (SDS-PAGE and HPLC)

assay

≥95% (HPLC)

assay

≥98% (SDS-PAGE and HPLC)

assay

≥95% (HPLC)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

predicted mol wt ~7.6 kDa

mol wt

~7.6 kDa

mol wt

7.5 kDa

mol wt

~7.6 kDa

packaging

glass bottle of 50 μg (in glass insert)

packaging

pkg of 100 μg, pkg of 50 μg

packaging

pkg of 5X50 μg, pkg of 50 μg

packaging

pkg of 50 and 100 μg

General description

Insulin-like growth factor I (IGF-I) belongs to the insulin-like growth factor (IGF; see MIM 147440) family of signaling molecules. IGF-I is also known as somatomedin. In the developing embryo IGF-I is expressed primarily by mesenchymal-derived cells.

Application

Insulin-like Growth Factor-I from mouse has been used:
  • for the preparation of growth factor cocktail
  • as a media supplement for mouse embryonic fibroblasts culture
  • as a media supplement to serve as a positive control to investigate
  • the effects of Angelica sinensis on myotubes

Biochem/physiol Actions

Insulin-like growth factor I (IGF-I) plays critical roles in cellular energy metabolism and in growth and development, especially prenatal growth.

IGF-I is secreted from the liver into circulation in a process regulated by pituitary growth hormone (GH) and so it mediates the growth-promoting activity of GH. After birth IGF-I expression in most extrahepatic tissues declines and hepatic expression of IGF-I becomes GH-regulated. Expression of IGF-I outside the liver is regulated differently, depending on the specific tissues. For example, gonadotropins and sex steroids regulate IGF-I expression within the reproductive system, while parathyroid hormone and sex steroids regulate IGF-I expression in bone. IGF-I is produced in several human tumors. IGF-I is mitogenic for a variety of cells including fibroblasts, osteoblasts, smooth muscle cells, fetal brain cells, neuroglial cells, and erythroid progenitor cells. IGF-I exerts its actions exclusively through the IGF-I receptor (IGF-IR). IGF-I induces endothelial cell migration and is involved in the regulation of angiogenesis.
Potent mitogenic growth factor that mediates the growth-promoting activities of growth hormone postnatally. Mouse and human IGF-I share 97% sequence identity.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.

Analysis Note

The biological activty is measured in a serum-free cell proliferation assay using the human breast carcinoma cell line, MCF-7.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Insulin-like Growth Factors (IGF)

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