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L6170

Sigma-Aldrich

β-Lactamase

recombinant, expressed in E. coli

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Synonym(s):
Carbapenemase, Cephalosprinase
CAS Number:
Enzyme Commission number:
EC Number:
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

form

powder

specific activity

≥20 U/mg (with cephalosporin C)
≥400 U/mg (with benzylpenicilin)

storage temp.

2-8°C

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This Item
P4524426205AB3738-I
vibrant-m

L6170

β-Lactamase

vibrant-m

AB3738-I

Anti-Beta Lactamase

recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

-

form

powder

form

lyophilized powder

form

lyophilized

form

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

-

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

General description

β-Lactamase produced by bacteria is closely related to the penicillin binding proteins. β-Lactamase hydrolyzes β-lactum antibiotics and is the prime cause of resistance development by bacteria. There are four subclasses of β-Lactamases. Classes A, C and D form an acyl-enzyme via active site serine residue. Class B β-lactamases are metalloenzymes with zinc ion at their active site for β-lactam hydrolysis. Mutations in the β-lactamases has resulted in the generation of extended-spectrum β-lactamases (ESBLs). As close to 900 types of β-Lactamases are produced by microbes.

Application

β--lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β--lactamase is used to study antibiotic resistance and resistance suppression. Product L6170 is recombinantly produced based on the sequence of the enzyme from Pseudomonas aeruginosa, and is expressed in E. coli.
β-Lactamase has been used in coumarin-cephalosporin-fluorescein-acetoxymethyl (CCF4)- β-Lactamase assay in dendritic cells and in the hydrolysis of meropenem (H-Mer) to generate 18O-labeled H-Mer.

Biochem/physiol Actions

β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring. Typical analysis for other substrates: ceftriaxon 2-4 un/mg; cefazolin 2-4 un/mg; ceftazidime 1.5-3 un/mg; cefoxitin 0.35-0.7 un/mg; cefepime 1.2-2.4 un/mg; cefuroxime 1.5-3 un/mg; cefotaxime 1-2 un/mg, oxacillin 4-8 un/mg; imipenem 1.2-2.4 un/mg; and meropenem 3-6 un/mg
Typical analysis for other substrates: ceftriaxon 2-4 un/mg; cefazolin 2-4 un/mg; ceftazidime 1.5-3 un/mg; cefoxitin 0.35-0.7 un/mg; cefepime 1.2-2.4 un/mg; cefuroxime 1.5-3 un/mg; cefotaxime 1-2 un/mg, oxacillin 4-8 un/mg; imipenem 1.2-2.4 un/mg; and meropenem 3-6 un/mg

Unit Definition

One unit will hydrolyze 1.0 μmole substrate per min (βI: benzylpenicillin; βII: cephalosporin C) in 50 mM phosphate buffer, pH 7.0 containing 10 μM zinc chloride at 25 ºC.

Physical form

Lyophilized powder containing sodium chloride and potassium phosphate.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Updated functional classification of beta-lactamases
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Determining carbapenemase activity with 18O labeling and targeted mass spectrometry
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Analytical Chemistry, 85(22), 11014-11019 (2013)
Antibiotic resistance and extended spectrum beta-lactamases: Types, epidemiology and treatment
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New broad-spectrum β-lactamases such as KPC enzymes and CTX-M-15 enzymes threaten to markedly reduce the utility of our armamentarium of β-lactam agents, even our most potent drugs, such as carbapenems. NXL104 is a broad-spectrum non-β-lactam β-lactamase inhibitor. In this evaluation

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