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P0389

Sigma-Aldrich

Penicillinase from Bacillus cereus

lyophilized powder, 1,500-3,000 units/mg protein (using benzylpenicillin)

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Synonym(s):
β-Lactamase I from Bacillus cereus, β-Lactamase II, Cephalosporinase, Penicillin amido-β-lactam hydrolase
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.54

biological source

Bacillus sp. (Bacillus cereus)

Quality Level

form

lyophilized powder

specific activity

1,500-3,000 units/mg protein (using benzylpenicillin)

composition

Protein, ~10%

technique(s)

activity assay: suitable

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

2-8°C

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This Item
P4524L6170S7651
Penicillinase from Bacillus cereus lyophilized powder, 1,500-3,000 units/mg protein (using benzylpenicillin)

P0389

Penicillinase from Bacillus cereus

Sigma-Aldrich

L6170

β-Lactamase

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

application(s)

-

form

lyophilized powder

form

lyophilized powder

form

powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

-

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

200

General description

Penicillinase from Bacillus cereus has been shown to contain three distinct species when purified and examined for homogeneity by sedimentation analysis, amino-terminal analysis, and vertical acrylamide gel electrophoresis.

Application

Penicillinase can be used to reduce levels of penicillin in patients with severe penicillin reactions.
Used in the production of penicillin.
Used in the production of penicillin. Penicillinase from Bacillus cereus has been used in a comparison study with penicillinase from Bacillus subtillis where the Michaelis constant, shape of the Ph/activity curve and immunological properties were shown to be significantly different.

Biochem/physiol Actions

Penicillinase specifically catalyzes the hydrolysis of β-lactam ring of penicillin.

Unit Definition

One unit will hydrolyze 1.0 μmole of indicated substrate per min at pH 7.0 at 25 °C. Sold as benzylpenicillin units. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

Physical form

Lyophilized powder containing phosphate and citrate buffer salts

Analysis Note

Protein determined by biuret.

Inhibitor

Product No.
Description
Pricing

pictograms

Health hazard

signalword

Danger

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Customers Also Viewed

Junichiro Marui et al.
Journal of bioscience and bioengineering, 110(1), 8-11 (2010-06-15)
Aspergillus oryzae penicillin biosynthetic genes were clustered. The penicillin production was positively regulated by VeA, a global gene regulator required for transcriptional expression of the penicillin biosynthetic genes. Overexpression of the biosynthetic genes by a strong promoter yielded a greater
M A van Opstal et al.
Journal of pharmaceutical and biomedical analysis, 8(1), 49-60 (1990-01-01)
An automated assay for the determination of penicillin in formulations suitable for use in pharmaceutical quality control is presented. The method is based on the classical iodometric penicillin assay which is incorporated in a flow injection analysis (FIA) system. The
Properties of penicillinase from Bacillus cereus 569.
J Imsande et al.
The Journal of biological chemistry, 245(9), 2205-2212 (1970-05-10)
A Comparison of the Properties of Penicillinase produced by Bacillus subtilis and Bacillus cereus with and without Addition of Penicillin
Manson, E., et al.
Microbiology, 11, 493-505 (1954)
Anaphylactic shock after therapy with penicillinase.
A L HYMAN
Journal of the American Medical Association, 169(6), 593-594 (1959-02-07)

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