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L6533

Sigma-Aldrich

Lectin from Dolichos biflorus (horse gram)

biotin conjugate, buffered aqueous solution

Synonym(s):

Dolichos biflorus agglutinin, DBA

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
Pricing and availability is not currently available.

conjugate

biotin conjugate

Quality Level

200

form

buffered aqueous solution

composition

Protein, ≥2.5 mg/mL biuret

extent of labeling

≥3 mol biotin per mol lectin

storage temp.

−20°C

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General description

Lectin from Dolichos biflorus (horse gram) is a heterotetrameric glycoprotein with a molecular mass of around 113 kDa. Dolichos biflorus agglutinin (DBA) is specific for terminal α-linked N-acetyl-D-galactosamine (GalNAc). It is a dietary lectin.[1]

Application

Lectin from Dolichos biflorus (horse gram) has been used:
  • to characterize mouse uterine natural killer (uNK) cells[2]
  • in double staining with lectins to localize nuclear factor-κB (NF-κB) activation[3]
  • as a species-specific gonocyte marker for the determination of donor cell colonization[4]
  • to label undifferentiated spermatogonia/cat testicular tissue[5]

Biochem/physiol Actions

DBA has anti-A1 human blood group specificity, useful for distinguishing between A1 and A2 blood types. DBA has strong anti-Cad activity, and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.
Dolichos biflorus agglutinin (DBA) possesses binding activity for which it is used as a marker binucleate cells and can indicate the state of their differentiation.[6] It serves as a label for primordial germ cells (PGCs), gonocytes, pre-spermatogonia and spermatogonia.[5]

Packaging

Sold on the basis of mg active conjugate.

Physical form

contains 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca2+

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000341048
0000341048
SLCR1116
SLCM0434
SLCP3065

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Spermatogonial stem cell organization in felid testis as revealed by Dolichos biflorus lectin
Escada-Rebelo S, et al.
Andrology, 4(6), 1159-1168 (2016)
Rajesh Dash et al.
JACC. Cardiovascular interventions, 11(2), 195-205 (2018-01-20)
The study investigated whether a dose response exists between myocardial salvage and the depth of therapeutic hypothermia. Cardiac protection from mild hypothermia during acute myocardial infarction (AMI) has yielded equivocal clinical trial results. Rapid, deeper hypothermia may improve myocardial salvage.
B Anne Croy et al.
Methods in molecular biology (Clifton, N.J.), 612, 465-503 (2009-12-25)
The term uterine natural killer (uNK) cell is applied in mice to an abundant but transient NK cell population that undergoes unique, terminal differentiation within embryo implantation sites during endometrial decidualization and pregnancy. In mice, decidualization is induced by attachment
Association of Dolichos biflorus lectin binding with full differentiation of bovine trophoblast cells
Nakano H, et al.
Reproduction (Cambridge, England), 124(4), 581-592 (2002)
Analysis of uterine natural killer cells in mice
Croy BA, et al.
Natural Killer Cell Protocols, 465-503 (2010)
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