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MAK066

Sigma-Aldrich

Lactate Dehydrogenase Activity Assay Kit

sufficient for 500 colorimetric tests

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Synonym(s):
LDH Activity Assay Kit, LDH Assay, Lactic Dehydrogenase Activity Assay Kit
NACRES:
NA.84

usage

sufficient for 500 colorimetric tests

detection method

colorimetric

relevant disease(s)

hematological disorder; cancer

storage temp.

−20°C

Gene Information

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storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

usage

sufficient for 500 colorimetric tests

usage

sufficient for 100 colorimetric tests

usage

-

usage

-

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

relevant disease(s)

hematological disorder; cancer

relevant disease(s)

cardiovascular diseases; cancer; hematological disorder

relevant disease(s)

neurological disorders

relevant disease(s)

neurological disorders

General description

Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate. Cells release LDH into the bloodstream after tissue damage or red blood cell hemolysis. Since LDH is a fairly stable enzyme, it has been widely used to evaluate the presence of damage and toxicity of tissue and cells. LDH is also elevated in certain pathological conditions such as cancer. Quantification of LDH has a broad range of applications.

Application

Lactate Dehydrogenase Activity Assay Kit has been used for measuring the activity of lactate dehydrogenase (LDH) in the cell culture medium.
Lactate Dehydrogenase Activity Assay Kit has been used to determine the concentration of lactate dehydrogenase in samples.

Suitability

Suitable for the detection of L(+)-Lactate in biological samples such as serum, plasma, cells, culture and fermentation media.

Principle

In this kit, LDH reduces NAD to NADH, which is specifically detected by colorimetric (450 nm) assay. The LDH Activity Assay kit quantifies LDH activity in variety of biological samples. The assay is quick, convenient, and sensitive.

Replaced by

pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3


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Food research international (Ottawa, Ont.), 97, 191-198 (2017-06-05)
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Chen Q, et al.
The Journal of Biological Chemistry, jbc-M115 (2015)

Articles

We presents an article about the Warburg effect, and how it is the enhanced conversion of glucose to lactate observed in tumor cells, even in the presence of normal levels of oxygen. Otto Heinrich Warburg demonstrated in 1924 that cancer cells show an increased dependence on glycolysis to meet their energy needs, regardless of whether they were well-oxygenated or not.

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