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MAK093

Sigma-Aldrich

Phosphofructokinase (PFK) Activity Colorimetric Assay Kit

sufficient for 100 colorimetric tests

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NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

cancer; orthopedic diseases; aging/geriatric diseases

storage temp.

−20°C

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SAB1400203SAB1401282SAB1300130
detection method

colorimetric

detection method

-

detection method

-

detection method

-

usage

sufficient for 100 colorimetric tests

usage

-

usage

-

usage

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

relevant disease(s)

cancer; orthopedic diseases; aging/geriatric diseases

relevant disease(s)

-

relevant disease(s)

-

relevant disease(s)

-

General description

Phosphofructokinase (PFK) catalyzes the third step of glycolysis, the conversion of fructose-6-phosphate to fructose-1,6-diphosphate, the rate limiting step of glycolysis. PFK activity is highly regulated by multiple cofactors in addition to post-translational modifications and can be used to measure glycolytic flux in tissues. Deficiencies in PFK activity can result in the glycogen storage disease, glycogenosis type VII (Tarui′s disease), which is a glycogen storage disease.

Application

Phosphofructokinase (PFK) Activity Colorimetric Assay Kit has been used to measure the activity of phosphofructokinase.

Suitability

Suitable for the measurement of phosphofructokinase activity, analysis of glucose metabolism and cell signaling and screening anti-cancer drugs in animal tissues (liver, brain, heart, muscles etc.) and cell culture (adherent or suspension cells).

Principle

The Phosphofructokinase Colorimetric Assay kit provides a simple and direct procedure for measuring PFK activity in a variety of samples. PFK activity is determined by a coupled enzyme assay, in which fructose-6-phosphate and ATP is converted to fructose-1,6-diphosphate and ADP by PFK. The ADP is converted by the enzyme mix to AMP and NADH. The resulting NADH reduces a colorless probe resulting in a colorimetric (450 nm) product proportional to the PFK activity present. One unit of PFK is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 7.4 at 37 °C.

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Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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