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Sigma-Aldrich

Mitochondrial Complex III Activity Assay Kit

sufficient for 100 colorimetric tests

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usage

sufficient for 100 colorimetric tests

detection method

colorimetric

storage temp.

−20°C

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MAK068MAK159MAK129
β-Glucosidase Activity Assay Kit sufficient for 100 colorimetric tests

MAK129

β-Glucosidase Activity Assay Kit

detection method

colorimetric

detection method

colorimetric

detection method

fluorometric

detection method

colorimetric

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

Mitochondrial Complex III or Ubiquinol-Cytochrome c reductase is the third complex of the Electron Transport Chain located in the mitochondrial membrane. It is present in the mitochondria of all aerobic eukaryotes as well as in the inner membranes of most bacteria. It transfers electrons from CoQH (reduced coenzyme Q or ubiquinol) to cytochrome c, resulting in the reduction of cytochrome c. This reduced cytochrome c is a substrate for complex IV of the electron transport chain.

Suitability

Suitable for the measurement of Mitochondrial Complex III activity in isolated mitochondria.

Principle

The Mitochondrial Complex III Activity Assay is based on the reduction of cytochrome c through the activity of Complex III. The absorbance of reduced cytochrome c can be measured at A550 nm. It is a fast and reliable method to determine the activity of complex III in isolated mitochondria. The kit can detect as low as 10 mU/mL and is linear up to 30 mU/mL.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

188.6 °F

flash_point_c

87 °C


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Lucie Aumailley et al.
Redox biology, 57, 102491-102491 (2022-10-01)
Ascorbate is a crucial antioxidant and essential cofactor of biosynthetic and regulatory enzymes. Unlike humans, mice can synthesize ascorbate thanks to the key enzyme gulonolactone oxidase (Gulo). In the present study, we used the Gulo-/- mouse model, which cannot synthesize

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