MAK360
Mitochondrial Complex III Activity Assay Kit
sufficient for 100 colorimetric tests
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 100 colorimetric tests
detection method
colorimetric
storage temp.
−20°C
General description
Mitochondrial Complex III or Ubiquinol-Cytochrome c reductase is the third complex of the Electron Transport Chain located in the mitochondrial membrane. It is present in the mitochondria of all aerobic eukaryotes as well as in the inner membranes of most bacteria. It transfers electrons from CoQH (reduced coenzyme Q or ubiquinol) to cytochrome c, resulting in the reduction of cytochrome c. This reduced cytochrome c is a substrate for complex IV of the electron transport chain.
Suitability
Suitable for the measurement of Mitochondrial Complex III activity in isolated mitochondria.
Principle
The Mitochondrial Complex III Activity Assay is based on the reduction of cytochrome c through the activity of Complex III. The absorbance of reduced cytochrome c can be measured at A550 nm. It is a fast and reliable method to determine the activity of complex III in isolated mitochondria. The kit can detect as low as 10 mU/mL and is linear up to 30 mU/mL.
Storage Class
10 - Combustible liquids
flash_point_f
188.6 °F
flash_point_c
87 °C
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Ahmed Ismaeel et al.
Nutrients, 14(5) (2022-03-11)
Previous studies have shown that chronic heavy alcohol consumption and consumption of a high-fat (HF) diet can independently contribute to skeletal muscle oxidative stress and mitochondrial dysfunction, yet the concurrent effect of these risk factors remains unclear. We aimed to
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Scientific reports, 11(1), 19408-19408 (2021-10-02)
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Redox biology, 57, 102491-102491 (2022-10-01)
Ascorbate is a crucial antioxidant and essential cofactor of biosynthetic and regulatory enzymes. Unlike humans, mice can synthesize ascorbate thanks to the key enzyme gulonolactone oxidase (Gulo). In the present study, we used the Gulo-/- mouse model, which cannot synthesize
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