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O1516

Sigma-Aldrich

Oil Red O solution

0.5% in propylene glycol

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Synonym(s):
Solvent Red 27, Sudan Red 5B
Empirical Formula (Hill Notation):
C26H24N4O
CAS Number:
Molecular Weight:
408.49
NACRES:
NA.47

form

liquid

Quality Level

concentration

0.5% in propylene glycol

λmax

0.40-1.00 at 506-520 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

InChI

1S/C26H24N4O/c1-16-9-10-17(2)22(13-16)27-28-23-14-19(4)24(15-18(23)3)29-30-26-21-8-6-5-7-20(21)11-12-25(26)31/h5-15,29H,1-4H3/b28-27?,30-26-

InChI key

HJAYEODIXUYVIC-LTFAAIOASA-N

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1 of 4

This Item
O1391O0625S4131
vibrant-m

O1516

Oil Red O solution

vibrant-m

O1391

Oil Red O solution

vibrant-m

O0625

Oil Red O

vibrant-m

S4131

Sudan III

form

liquid

form

liquid

form

powder

form

powder

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

λmax

0.40-1.00 at 506-520 nm

λmax

-

λmax

-

λmax

-

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

General description

Oil Red O stains and imparts red-orange color to lipids. It has also been shown to mark vacuoles, observed in acute lymphoblastic leukemia (ALL-L3) or Burkitt′s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids.

Application

Oil red O solution has been used to identify intracytoplasmatic lipid droplets on 3T3-L1 cell line adipocytes and to measure lipid content in Simpson-Golabi-Behmel syndrome (SGBS) preadipocytes.
Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

217.4 °F

flash_point_c

103 °C


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