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1.05230

Sigma-Aldrich

Oil red O (C.I. 26125)

for microscopy Certistain®

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Synonym(s):
Oil red O (C.I. 26125)
CAS Number:
Molecular Weight:
408.5
MDL number:
EC Index Number:
215-295-3

composition

Dye, ≥80% spectrophotometric assay

mp

182-186 °C

bulk density

270 kg/m3

εmax

725-910 at 528-534 nm in chloroform at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/C26H24N4O/c1-16-9-10-17(2)22(13-16)27-28-23-14-19(4)24(15-18(23)3)29-30-26-21-8-6-5-7-20(21)11-12-25(26)31/h5-15,29H,1-4H3/b28-27?,30-26-

InChI key

HJAYEODIXUYVIC-LTFAAIOASA-N

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This Item
O0625O1391O1516
vibrant-m

1.05230

Oil red O (C.I. 26125)

vibrant-m

O0625

Oil Red O

vibrant-m

O1391

Oil Red O solution

vibrant-m

O1516

Oil Red O solution

mp

182-186 °C

mp

120 °C (dec.) (lit.)

mp

-

mp

-

εmax

725-910 at 528-534 nm in chloroform at 0.01 g/L

εmax

-

εmax

0.20-0.50 at 510-517 nm

εmax

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

bulk density

270 kg/m3

bulk density

-

bulk density

-

bulk density

-

General description

The dry dye Oil red O (C.I. 26125) for microscopy Certistain®, is used for the preparation of a staining solution that can be used for the detection of lipids in histological cryo sections. As a Certistain® dye it is chemically quality-certified according to highest standards and strict specifications. Oil red O staining is used for the detection of neutral fat or polyethylene granula in e. g. histological tissues, in joint pathology or in cytological sample material. This Oil red O solution is ready-to-use and results in a brilliant, bright red staining of lipids, while the nuclei are counterstained blue with Hematoxylin solution acc. to Gill II (Cat. no. 105175).
The staining of lipids in histology specimens is a physical process. Oil red O is a lipophilic dye; due to its solubility characteristics it diffuses to the tissue lipids. It is registered as IVD and certified as CE product. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Application

Certistain® dry dye used to detect lipids in cryosections

Analysis Note

Dye content (spectrophotometrically)≥ 80 %Identity (UV/VIS-Spectrum)passes testAbsorption maximum λ max (chloroform)528 - 534 nmSpec. Absorptivity A 1%/1cm (λmax; 0.01 g/l; chloroform)725 - 910TLC-Testpasses testLoss on drying (110 °C)≤ 8 %Suitability for microscopypasses test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

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