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P4020

Sigma-Aldrich

Protamine sulfate salt from salmon

Grade X, amorphous powder

Synonym(s):
Salmine, Salmine from salmon
CAS Number:
MDL number:
NACRES:
NA.77

type

Grade X

Quality Level

form

amorphous powder

impurities

histone, free (Millon test)

color

white to off-white

solubility

6 M HCl: 25 mg/mL

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P6188P4380P0424
form

amorphous powder

form

powder

form

powder

form

powder

color

white to off-white

color

white to off-white

color

white to off-white

color

white

solubility

6 M HCl: 25 mg/mL

solubility

H2O: 1 mg/mL (Hydrolyzes to 6-ketoprostaglandin F in aqueous solution.), ethanol: 50 mg/mL

solubility

6 M HCl: 25 mg/mL, clear, colorless to light yellow

solubility

H2O: 1 mg/mL (Aqueous solutions are stable for 30 days at 2-8°C and for several months frozen at −0°C in single use aliquots. Avoid repeated freeze/thaw cycles.), ethanol: 50 mg/mL

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

type

Grade X

type

-

type

Grade II

type

-

impurities

histone, free (Millon test)

impurities

-

impurities

-

impurities

-

General description

Protamine are proteins rich in cysteine and arginine. It possesses many phosphorylation sites. It is found to be localized to the sperm head.

Application

Protamine sulfate salt from salmon has been used:
  • in the pretreament of cells to study its effect on respiratory syncytial virus infection
  • in lentivirus production
  • to derivatize it into a diazeniumdiolate NO donor

May be used for the removal of DNA from protein samples or for the purification of DNA binding proteins. It may be used as an alternative to polybrene in retroviral-mediated gene transfer.

Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.
Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.

Biochem/physiol Actions

Protamine sulfate is a small cationic protein that binds and precipitates DNA. Neutralizes the anticoagulant activity of heparin. Inhibits lipoprotein lipase.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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