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P7130

Sigma-Aldrich

Puromycin aminonucleoside

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Synonym(s):
3′-Amino-3′-deoxy-N6,N6-dimethyladenosine
Empirical Formula (Hill Notation):
C12H18N6O3
CAS Number:
Molecular Weight:
294.31
Beilstein:
93902
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.85

form

powder

Quality Level

solubility

H2O: soluble 50 mg/mL

antibiotic activity spectrum

Gram-positive bacteria
neoplastics
parasites

Mode of action

protein synthesis | interferes

storage temp.

2-8°C

SMILES string

CN(C)c1ncnc2n(cnc12)[C@@H]3O[C@H](CO)[C@H](N)[C@@H]3O

InChI

1S/C12H18N6O3/c1-17(2)10-8-11(15-4-14-10)18(5-16-8)12-9(20)7(13)6(3-19)21-12/h4-7,9,12,19-20H,3,13H2,1-2H3/t6-,7+,9+,12-/m1/s1

InChI key

RYSMHWILUNYBFW-VENHTOENSA-N

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This Item
SBR00017P8833P9620
solubility

H2O: soluble 50 mg/mL

solubility

-

solubility

H2O: soluble 50 mg/mL, clear, colorless to faintly yellow

solubility

H2O: soluble 10 mg/mL

antibiotic activity spectrum

Gram-positive bacteria, neoplastics, parasites

antibiotic activity spectrum

Gram-positive bacteria, neoplastics, parasites

antibiotic activity spectrum

Gram-positive bacteria, neoplastics, parasites

antibiotic activity spectrum

Gram-positive bacteria, neoplastics, parasites

mode of action

protein synthesis | interferes

mode of action

protein synthesis | interferes

mode of action

protein synthesis | interferes

mode of action

protein synthesis | interferes

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

300

Quality Level

200

Quality Level

300

Quality Level

300

General description

Puromycin aminonucleoside is the aminonucleoside portion of the antibiotic puromycin. It is useful in nephrology research, like studies of focal and segmental glomerulosclerosis and in the induction of nephrosis in rats. The excretion of sodium and nitrite (NOx) metabolites in rats with puromycin aminonucleoside-induced nephrotic syndrome are studied. Puromycin aminonucleoside-induced nephrosis in rats has been studied with respect to the production of reactive oxygen species during the acute phase. Puromycin aminonucleoside is used to probe endothelial glycosaminoglycan synthesis in cultured glomerular endothelial cells and their relation to cell permeability.

Application

Puromycin aminonucleoside has been used:
  • as a selection marker for the infected cells for the transfection of lentivirus
  • as a component of fresh medium for transfection and infection assays
  • to subcutaneously induce puromycin aminonucleoside nephrosis to investigate the mRNA and protein levels of nephrin and podocin before the onset of proteinuria

Biochem/physiol Actions

Puromycin aminonucleoside is used to study human glomerular disease by inducing damage of murine glomerular podocytes and is used to study glomerular function and morphology.

Other Notes

Keep container tightly closed in a dry and well-ventilated place.

Pictograms

Health hazard

Signal Word

Warning

Hazard Statements

Hazard Classifications

STOT RE 2

Target Organs

Kidney

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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