MilliporeSigma
All Photos(2)

PHG0005

SAFC

BIS-TRIS propane

Synonym(s):
1,3-Bis[tris(hydroxymethyl)methylamino]propane
Linear Formula:
CH2[CH2NHC(CH2OH)3]2
CAS Number:
Molecular Weight:
282.33
Beilstein:
1786109
EC Number:
MDL number:
NACRES:
NA.21

biological source

synthetic

Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, microbial, and trace metals; tested

useful pH range

6.3-9.5

pKa (25 °C)

(1) 6.8, (2) 9.0

mp

164-165 °C (lit.)

suitability

suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

SMILES string

OCC(CO)(CO)NCCCNC(CO)(CO)CO

InChI

1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2

InChI key

HHKZCCWKTZRCCL-UHFFFAOYSA-N

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B4679B6755B9410
BIS-TRIS propane

SAFC

PHG0005

BIS-TRIS propane

BIS-TRIS propane BioPerformance Certified, suitable for cell culture, ≥99.0%

Sigma-Aldrich

B4679

BIS-TRIS propane

BIS-TRIS propane ≥99.0% (titration)

Sigma-Aldrich

B6755

BIS-TRIS propane

BIS-TRIS propane BioXtra, ≥99.0% (titration)

Sigma-Aldrich

B9410

BIS-TRIS propane

form

powder

form

crystals

form

powder

form

powder

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

impurities

Endotoxin, microbial, and trace metals; tested

impurities

endotoxin and bioburden, tested

impurities

-

impurities

≤0.005% Phosphorus (P), ≤0.1% Insoluble matter

mp

164-165 °C (lit.)

mp

164-165 °C (lit.)

mp

164-165 °C (lit.)

mp

164-165 °C (lit.)

suitability

suitable for manufacturing use

suitability

-

suitability

-

suitability

-

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

foreign activity

DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected

foreign activity

-

foreign activity

-

General description

Our SAFC® portfolio of high-quality raw materials for use in biopharmaceutical processing withstands strict quality control procedures plus the documentation and expertise to help our customers meet requirements as defined by the M-Clarity Program.

M-Clarity Program

Buffer quality is vital for the success of biopharmaceutical processes, because buffers are indispensable in nearly every production step.

Our broad portfolio of buffer materials manufactured under appropriate controls is tailored to your needs. Ranging from non-GMP grades for low-risk application, to IPEC-PQG GMP for higher-risk applications, we have products covering all your manufacturing needs.

Application

Bis Tris Propane is a unique biological buffer also referred to as a “Universal” buffer, which means the buffer capacity covers a wide range. Bis Tris Propane has two pKas of 6.3 and 9.5, which expands the buffering range from 5.0-10.5-making this a versatile buffer for biological systems. Bis Tris Propane is considered to be non-toxic to culture cell lines and provides high-solution clarity.

A review of DNA polymerase techniques lists bis tris propane as a suitable buffer for PCR methods as a universal buffer pH gradient is employed.

Legal Information

SAFC is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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