MilliporeSigma
All Photos(2)

PHG0004

SAFC

BIS-TRIS

Synonym(s):
2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane
Empirical Formula (Hill Notation):
C8H19NO5
CAS Number:
Molecular Weight:
209.24
Beilstein:
2205275
EC Number:
MDL number:
NACRES:
NA.21

biological source

synthetic

Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, microbial, and trace metals; tested

useful pH range

5.8-7.2

pKa (25 °C)

6.5

solubility

H2O: 0.1 M, clear, colorless

suitability

suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

SMILES string

OCCN(CCO)C(CO)(CO)CO

InChI

1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2

InChI key

OWMVSZAMULFTJU-UHFFFAOYSA-N

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This Item
B9754137260137276
BIS-TRIS

SAFC

PHG0004

BIS-TRIS

BIS-TRIS ≥98.0% (titration)

Sigma-Aldrich

B9754

BIS-TRIS

BIS-TRIS EMPROVE® EVOLVE

SAFC

137260

BIS-TRIS

BIS-TRIS EMPROVE® EVOLVE

SAFC

137276

BIS-TRIS

form

powder

form

crystalline powder

form

powder

form

powder

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, microbial, and trace metals; tested

impurities

-

impurities

Endotoxin, microbial, and trace metals, tested

impurities

Endotoxin, microbial, and trace metals; tested

solubility

H2O: 0.1 M, clear, colorless

solubility

H2O: 500 mg/mL, clear, colorless to very faintly yellow

solubility

water: 0.1 M, clear, colorless (aqueous)

solubility

water: 0.1 M, clear, colorless

suitability

suitable for manufacturing use

suitability

-

suitability

suitable for manufacturing use

suitability

suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

foreign activity

-

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

foreign activity

-

General description

Our SAFC® portfolio of high-quality raw materials for use in biopharmaceutical processing withstands strict quality control procedures plus the documentation and expertise to help our customers meet requirements as defined by the M-Clarity Program.

M-Clarity Program

Buffer quality is vital for the success of biopharmaceutical processes, because buffers are indispensable in nearly every production step.

Our broad portfolio of buffer materials manufactured under appropriate controls is tailored to your needs. Ranging from non-GMP grades for low-risk application, to IPEC-PQG GMP for higher-risk applications, we have products covering all your manufacturing needs.

Application

Bis Tris is a commonly used biological buffer for very broad usage in biological systems such as diagnostics, electrophoresis and protein based bioprocessing. The pKa of Bis Tris is 6.36 which makes it a good candidate for biological-based formulations.

Bis Tris is used in a wide variety of biological applications including protein separation buffers, cell culture media, electrophoresis separations and diagnostic reagent formulations.

Legal Information

SAFC is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Dam. 1

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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