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RAB0874

Sigma-Aldrich

Porcine INS / Insulin ELISA Kit

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NACRES:
NA.32

species reactivity

porcine

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 4 μIU/mL
standard curve range: 4.69 μIU/ml-300 μIU/ml

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

Porcine ... INS(397415)

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RAB0874

Porcine INS / Insulin ELISA Kit

vibrant-m

RAB1134

Porcine OPG ELISA Kit

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 4 μIU/mL
standard curve range: 4.69 μIU/ml-300 μIU/ml

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 6 pg/mL
standard curve range: 8.23-6000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.12 ng/ml

assay range

inter-assay cv: <10%
intra-assay cv: <12%

Gene Information

Porcine ... INS(397415)

Gene Information

Porcine ... IL1B(397122)

Gene Information

Porcine ... TNFRSF11B(100738370)

Gene Information

human ... INSR(3643)

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum

input

sample type plasma
sample type serum sample(s)
sample type cell culture supernatant(s)

input

sample type: human cell lysate

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

General description

The antibody reacts specifically against insulin by RIA and immunocytochemistry. It exhibits cross-reactivity with human proinsulin. The antibody binds to insulin with an affinity constant of 8.8 × 109 M-1 in RIA. The insulin gene is mapped to human chromosome 11p15.5.
Human insulin is made up of 51 amino acids. It consists of two peptide chains, 21 amino acids make up A-chain and chain B contains 31 amino acids. The two chains are linked together by a disulfide bond, to form a classical insulin fold with three α helices, chain A having two and one in chain B.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Abnormal insulin secretion or function, leads to diabetes mellitus, obesity and other carbohydrate metabolism disorders. Insulin is known to stimulate cell division. The biological effects of insulin are mediated by insulin receptor and it integrates major signaling pathways.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Genetic linkage of Beckwith-Wiedemann syndrome to 11p15.
Ping A J, et al.
American Journal of Human Genetics, 44(5), 720-720 (1989)
Kinetic properties of monoclonal insulin antibodies.
Keilacker H, et al.
Biomedica Biochimica Acta, 45(8), 1093-1102 (1986)
Proinsulin-Specific Monoclonal Antibodies: Immunocytochemical Application as ?-Cell Markers and as Probes for Conversion.
Madsen O D
Diabetes, 36(10), 1203-1211 (1987)
S Witt et al.
Acta histochemica. Supplementband, 35, 217-223 (1988-01-01)
Murine monoclonal antibodies against glucagon and insulin were generated by somatic cell hybridization and partially characterized. The monoclonal glucagon antibody K79bB10 exhibited no cross-reaction with gut glucagon. This antibody and the insulin antibody K36aC10 were found of very high concentration
The molecular mechanism of insulin action.
Kahn C R
Annual Review of Medicine, 36(1), 429-451 (1985)

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