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RES3098T-B7

SAFC

TRIS hydrochloride

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Synonym(s):
Trizma® hydrochloride, TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
1185-53-1
Molecular Weight:
157.60
Beilstein:
3675235
EC Number:
214-684-5
MDL number:
MFCD00012590
NACRES:
NA.21

biological source

synthetic

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, microbial, and trace metals, tested

useful pH range

7.0-9.0

pKa (25 °C)

8.1

suitability

suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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This Item
PHG00029310-OPT15760
TRIS hydrochloride

SAFC

RES3098T-B7

TRIS hydrochloride

TRIS hydrochloride

SAFC

PHG0002

TRIS hydrochloride

OmniPur® TRIS Hydrochloride

Millipore

9310-OP

OmniPur® TRIS Hydrochloride

Trizma® hydrochloride reagent grade, ≥99%, crystalline

Sigma-Aldrich

T15760

Trizma® hydrochloride

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

impurities

Endotoxin, microbial, and trace metals, tested

impurities

Endotoxin, microbial, and trace metals; tested

impurities

-

impurities

-

suitability

suitable for manufacturing use

suitability

suitable for manufacturing use

suitability

-

suitability

-

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

foreign activity

-

foreign activity

-

biological source

synthetic

biological source

synthetic

biological source

-

biological source

synthetic

Application

Tris Hydrochloride is a commonly used biological buffer which is also referred to as Trizma HCl. Tris as a buffer has very broad usage in biological systems such as diagnostics, downstream purification and cell culture. The pKa of Tris is 7.77 which corresponds closely to a physiological pH of 7.36.

Tris HCl s used in biopharmaceutical buffer formulations (both upstream and downstream), electrophoresis separations and diagnostic reagent formulations. Tris HCl is especially useful in downstream purification processes.

Packaging

Product is available in the following package sizes:
RES3098T-B701X: 100g container
RES3098T-B703X: 5kg container
RES3098T-B705X: 25kg container
RES3098T-B706X: 50kg container

Other Notes

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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25G
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Tris, Hydrochloride, Molecular Biology Grade Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.

Millipore

648317

Tris, Hydrochloride, Molecular Biology Grade

Tris base =99.9% (titration), abs., 100 mg/mL, 300 nm ≤0.015

Sigma-Aldrich

TRIS-RO

Tris base

Tris(hydroxymethyl)aminomethane hydrochloride EMPROVE® EXPERT

SAFC

1.08219

Tris(hydroxymethyl)aminomethane hydrochloride

Tris, Hydrochloride, ULTROL® Grade Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C. Absorbance (1.0 M, H2O, 260 nm):≤0.1.

Millipore

648313

Tris, Hydrochloride, ULTROL® Grade

Tromethamine

SAFC

RES3193T-A7

Tromethamine

Sigma-Aldrich

Sigma-Aldrich

12-457

MYPT1 (654-880)

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