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About This Item

Empirical Formula (Hill Notation):
C27H29N2NaO7S2
CAS Number:
3520-42-1
Molecular Weight:
580.65
Colour Index Number:
45100
Beilstein/REAXYS Number:
3886008
EC Number:
222-529-8
MDL number:
MFCD00010180
UNSPSC Code:
12352207
PubChem Substance ID:
24899477
NACRES:
NA.75
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biological source

synthetic (organic)

Quality Level

100

product line

BioReagent

form

powder

composition

Dye content, ~75%

technique(s)

cell culture | mammalian: suitable

solubility

methanol: 1 mg/mL

fluorescence

λex 565 nm; λem 586 nm in H2O

SMILES string

[Na+].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S([O-])(=O)=O)=[N+](\CC)CC)c1

InChI

1S/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1

InChI key

SXQCTESRRZBPHJ-UHFFFAOYSA-M

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General description

Sulforhodamine B is a rhodamine derived aminoxanthene dye. It contains two sulfonic acids groups which separate in alkaline condition.

Application

Sulforhodamine B sodium salt has been used:
  • for relative total cell mass quantification
  • to investigate the viscosity of coacervates
  • as a molecular rotor for microviscosity determination in aerosol droplets

Biochem/physiol Actions

Sulforhodamine B sodium salt is useful to measure cell biomass and also for cytotoxicity screening.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBR3296
SHBN2223
SHBL9542
MKCD3873
MKCD1019

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