230162

Sulforhodamine B

Dye content 75 %

• Synonym(s): Acid Red 52, Sulforhodamine B monosodium salt
• Empirical Formula (Hill Notation): C₂₇H₂₉N₂NaO₇S₂
• CAS Number: 3520-42-1
• Molecular Weight: 580.65
• Colour Index Number: 45100
• Beilstein/REAXYS Number: 3886008
• EC Number: 222-529-8
• MDL number: MFCD00010180
• UNSPSC Code: 12171500
• PubChem Substance ID: 24853778
• NACRES: NA.47

Properties

• assay: ≥73% (HPLC)
• form: powder
• composition: Dye content, 75%
• technique(s): microbe id | staining: suitable
• color: brown to very dark brown; green to very dark green
• solubility: methanol: 1 mg/mL
• λmax: 554 nm
• ε (extinction coefficient): ≥85000 at 554-560 nm in 0.1 M NaOH in methanol at 0.005 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S([O-])(=O)=O)=[N+](\CC)CC)c1
• InChI: 1S/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1
• InChI key: SXQCTESRRZBPHJ-UHFFFAOYSA-M

Description

General description

Sulforhodamine B (SRB) is a bright-pink aminoxanthene dye. The sulforhodamine B (SRB) assay is extensively used for in vitro cytotoxicity screening. SRB binds to the protein constituents of the cells in a stoichiometric manner. Thus, the amount of dye extracted from stained cells is directly proportional to the cell mass. SRB can also be used for cell density determination.

Application

Sulforhodamine B has been used as a dye in the MCF-7 (human breast adenocarcinoma cell line) cell proliferation assay.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)