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SAB3700096

Sigma-Aldrich

Anti-Dog IgG F(ab′)2-Peroxidase antibody produced in rabbit

affinity isolated antibody, lyophilized powder

Synonym(s):

HRP

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25 MG
$74.80
100 MG
$221.00
500 MG
$649.44
1 G
$941.60

$74.80


Availability

Available to ship onJanuary 09, 2025Details



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25 MG
$74.80
100 MG
$221.00
500 MG
$649.44
1 G
$941.60

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

$74.80


Availability

Available to ship onJanuary 09, 2025Details


biological source

rabbit

Quality Level

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

dog

technique(s)

immunohistochemistry: suitable
indirect ELISA: suitable
western blot: suitable

shipped in

wet ice

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This Item
P7890P6516P1399
technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

color

white to off-white

color

white to off-white

color

white to off-white

color

white to off-white

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

mol wt

≥300,000

mol wt

15,000-30,000 by viscosity

mol wt

≤15,000 by MALLS, 4,000-15,000 by viscosity

mol wt

150,000-300,000

General description

An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. In dogs four subclasses of IgG (IgG1 -IgG4) are present. Anti-Dog IgG F(ab’)2 antibody is ideal for investigators in Immunology, Cancer, and Microbiology research. Anti-Dog IgG F(ab′)2 Peroxidase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Peroxidase, Anti-Rabbit Serum, Dog IgG, Dog IgG F(ab′)2 and Dog Serum. No reaction was observed against Dog IgG F(c).

Immunogen

Dog IgG F(ab′)2 fragment

Physical properties

Antibody format: IgG

Physical form

Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstitution

Reconstitute with 1.0 mL deionized water (or equivalent).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Specific IgG for cat allergens in patients with allergic conjunctivitis
Miyama A, et al.
International Ophthalmology (2015)

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