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SAB3701083

Sigma-Aldrich

Anti-Mouse IgG (H+L)-Peroxidase antibody produced in rabbit

affinity isolated antibody, lyophilized powder

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Synonym(s):
HRP
NACRES:
NA.46

biological source

rabbit

Quality Level

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

mouse

technique(s)

immunohistochemistry: suitable
indirect ELISA: suitable
western blot: suitable

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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This Item
SAB3701066SAB3700920SAB3700248
antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

technique(s)

immunohistochemistry: suitable, western blot: suitable, indirect ELISA: suitable

technique(s)

immunohistochemistry: suitable, indirect ELISA: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, indirect ELISA: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, indirect ELISA: suitable, western blot: suitable

species reactivity

mouse

species reactivity

mouse

species reactivity

rabbit

species reactivity

goat

biological source

rabbit

biological source

goat

biological source

sheep

biological source

rabbit

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

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General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. It consists of a γ heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical (called Fab fragments) and contain the antigen-binding activity. The third fragment (called Fc fragment) does not possess antigen-binding activity, but binds to cells and effector molecules. Pepsin cleaves the carboxy-terminal side of the disulfide bonds in the general region of the antibody and this gives rise to the F(ab′)2 fragment. The two antigen-binding arms of the antibody are linked in this fragment. Maternal IgG is the only antibody transported across the placenta to the fetus. It passively immunizes the infants.

Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Peroxidase, Anti-Rabbit Serum, Mouse IgG and Mouse Serum.

Immunogen

Mouse IgG whole molecule

Physical properties

Antibody format: IgG

Physical form

Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstitution

Reconstitute with 1.0 mL deionized water (or equivalent).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Exclamation mark

signalword

Warning

hcodes

pcodes

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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