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SAB4200707

Sigma-Aldrich

Anti-Desmin antibody, Mouse monoclonal

enhanced validation

clone DE-U-10, purified from hybridoma cell culture

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Synonym(s):
Anti-DES
NACRES:
NA.41

antibody form

purified from hybridoma cell culture

Quality Level

antibody product type

primary antibodies

clone

DE-U-10, monoclonal

form

buffered aqueous solution

mol wt

antigen ~55 kDa

species reactivity

rat, rabbit, hamster, porcine, goat, bovine, chicken, toad, sheep, mouse, human

enhanced validation

independent
Learn more about Antibody Enhanced Validation

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 0.5-1 μg/mL using whole extract of mouse myoblast C2C12 cells
immunofluorescence: 2-5 μg/mL using mouse myoblast C2C12 cells
immunohistochemistry: 2.5-5 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded Human Appendix
microarray: suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DES(1674)
mouse ... Des(13346)
rat ... Des(64362)

General description

Anti-Desmin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma DE-U-10 produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/C mouse. Desmin, a major intermediate filament protein in skeletal, cardiac and smooth muscle cells. It is predominantly localised to filamentous extrasarcomeric cytoskeleton, which links neighbouring myofibrils and connects the myofibrillar apparatus with the subsarcolemmal cytoskeleton, myonuclei and mitochondria.

Immunogen

Desmin purified from pig stomach

Application

Anti-Desmin antibody, Mouse monoclonal has been used in:
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry
  • microarray

Biochem/physiol Actions

Mutations is the desmin gene (known as desminopathy or DRM) are associated with several human diseases such as atrophy and facial muscle weakness, cardiomyopathy, cardiac arrhythmias and restrictive heart failure and are characterized by intracellular desmin aggregates. Anti-Desmin antibodies can be implemented for cancer research and diagnostics since elevated levels of desmin characterize several types of muscle tissue tumors, such as leiomyomas and rhabdomyosarcomas. The antibody may also be used for meat research measurements.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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