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SAB5600116

Sigma-Aldrich

Anti-HA-Tag antibody, Rabbit Monoclonal

recombinant, expressed in HEK 293 cells, clone RM305, purified immunoglobulin

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Synonym(s):
Human influenza hemagglutinin amino acids 98-106
NACRES:
NA.41

recombinant

expressed in HEK 293 cells

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

RM305, monoclonal
recombinant monoclonal

form

buffered aqueous glycerol solution

technique(s)

flow cytometry: 0.1-1 μg/mL
immunocytochemistry: 0.1-1 μg/mL
immunohistochemistry: 0.01-0.5 μg/mL
immunoprecipitation (IP): 0.5-2 μg/mL
western blot: 0.1-1 μg/mL

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

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H6908H9658H3663
Anti-HA antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

H6908

Anti-HA antibody produced in rabbit

Anti-HA antibody, Mouse monoclonal clone HA-7, purified from hybridoma cell culture

H3663

Anti-HA antibody, Mouse monoclonal

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

ascites fluid

antibody form

purified immunoglobulin

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

technique(s)

flow cytometry: 0.1-1 μg/mL, immunohistochemistry: 0.01-0.5 μg/mL, western blot: 0.1-1 μg/mL, immunocytochemistry: 0.1-1 μg/mL, immunoprecipitation (IP): 0.5-2 μg/mL

technique(s)

immunoprecipitation (IP): 2.5-4 μg/test using HA-tagged fusion protein from cell lysates, indirect immunofluorescence: 10-20 μg/mL using HA-tagged fusion protein transfected cells, western blot: 0.5-0.8 μg/mL using HA-tagged fusion protein transfected cell extracts

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 1:20,000 using extracts from 293T (human embryonic kidney) cells expressing a recombinant HA-fusion protein

technique(s)

immunocytochemistry: 1.0-2.0 μg/mL, immunoprecipitation (IP): 0.5-2.0 μg, western blot: 0.25-1 μg/mL

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

form

buffered aqueous glycerol solution

form

buffered aqueous solution

form

-

form

-

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General description

The hemagglutinin (HA) gene is mapped to segment 4 of Influenza B viral genome. HA is a dominant antigen present on the influenza viral surface. It is a homotrimer and each monomer is produced as a single polypeptide.

Specificity

This antibody reacts to recombinant proteins containing a HA-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein.

Immunogen

A peptide corresponding to HA-tag (Human influenza hemagglutinin amino acids 98-106).

Application

Anti-HA-Tag antibody, Rabbit Monoclonal has been used in western blotting.

Biochem/physiol Actions

Hemagglutinin (HA) regulates membrane fusion and receptor-binding functions during viral entry and infection. The virus gains entry into the host cell via endocytosis and successive membrane fusion mediated by the HA antigen. HA plays a crucial role in viral pathogenesis and host response to viral infection.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Solution in phosphate buffered saline containing 50% glycerol, 1% BSA and 0.09% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Entry, Replication, Immune Evasion, and Neurotoxicity of Synthetically Engineered Bat-Borne Mumps Virus
Kruger N, et al.
Cell Reports, 25(2), 312-320 (2018)
Sandi Shen et al.
Biology, 12(4) (2023-04-28)
We previously demonstrated that mice with targeted deletion of the leucine repeat rich kinase 1 (Lrrk1) gene were osteopetrotic due to the failure of osteoclasts to resorb bone. To determine how LRRK1 regulates osteoclast activity, we examined the intracellular and
On the origin of the human influenza virus subtypes H2N2 and H3N2.
Scholtissek C, et al.
Virology, 87(1), 13-20 (1978)
Structure and receptor specificity of the hemagglutinin from an H5N1 influenza virus.
Stevens J, et al.
Science, 312(5772), 404-410 (2006)
Nadine Krüger et al.
Cell reports, 25(2), 312-320 (2018-10-12)
Bats harbor a plethora of viruses with an unknown zoonotic potential. In-depth functional characterization of such viruses is often hampered by a lack of virus isolates. The genome of a virus closely related to human mumps viruses (hMuV) was detected in

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