SBR00039

Hygromycin B

Min. 95% (HPLC ELSD) Ready Made Solution, 50 mg/mL in water (from Streptomyces hygroscopicus)

• Synonym(s): Hygromycin B solution from Streptomyces hygroscopicus
• Empirical Formula (Hill Notation): C₂₀H₃₇N₃O₁₃
• CAS Number: 31282-04-9
• Molecular Weight: 527.52
• Beilstein/REAXYS Number: 6755837
• MDL number: MFCD06795479
• UNSPSC Code: 51102829
• NACRES: NA.76

Properties

• product name: Hygromycin B, Min. 95% (HPLC ELSD) Ready Made Solution, 50 mg/mL in water (from Streptomyces hygroscopicus)
• biological source: Streptomyces hygroscopicus
• Quality Level: 200
• assay: ≥95%
• form: liquid
• concentration: 50 mg/mL in water (from Streptomyces hygroscopicus)
• antibiotic activity spectrum: Gram-negative bacteria; Gram-positive bacteria; fungi; viruses
• mode of action: protein synthesis | interferes
• storage temp.: 2-8°C
• SMILES string: CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3OC4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O
• InChI: 1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1
• InChI key: GRRNUXAQVGOGFE-XKIAHZFYSA-N

Description

General description

Hygromycin B, also known as Hydrovetine and D-streptamine, is a distinctive aminoglycoside antibiotic recognized for its ability to impede the growth of both prokaryotic microorganisms, such as bacteria, and eukaryotic microorganisms like yeasts and mammalian cells. Its mechanism of action involves reinforcing the interaction of peptidyl-tRNA in the ribosomal acceptor ("A") site, ultimately hindering the translocation process mediated by EF-2 (elongation Factor-2). Derived from Streptomyces hygroscopicus, this aminoglycoside antibiotic serves as a valuable selection agent for cells harboring the hygromycin-resistant gene, known as hph or hyg.

Widely utilized for selection and maintenance purposes, Hygromycin B showcases its inhibitory prowess on protein synthesis, contributing to its role in the selection and upkeep of both prokaryotic and eukaryotic cells featuring the hygromycin resistance gene. This antibiotic demonstrates anthelmintic activity in addition to its impact on translational processes in ribosomes across diverse organisms. Its specificity in inhibiting translocation, as well as its influence on decoding fidelity, makes Hygromycin B a valuable tool for the selection of transfected cells containing the Escherichia coli hygromycin resistance gene (hyg or hph) and for maintaining the Hygromycin phenotype in resistant cells. The monophasic inhibition of polypeptide synthesis suggests a single-site binding of Hygromycin B to the ribosome, adding a layer of specificity to its mode of action, making it indispensable for various facets of scientific research in cell biology and biochemical research.

Application

Hygromycin B. has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreadin. as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/m. specifically at 100 μg/mL for prokaryote. 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes

Biochem/physiol Actions

Mode of Action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.

Mode of action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.

Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.

Features and Benefits

• High-quality antibiotic suitable for multiple research applications
• Ideal for Cell Biology, Metabolomics, and Biochemical research
• Ready-made solution for ease of use
  

Other Notes

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Safety Information

• pictograms: GHS06
• signalword: Danger
• hcodes: H301,H312,H330
• pcodes: P260 - P264 - P270 - P280 - P302 + P352 + P312 - P304 + P340 + P310
• Hazard Classifications: Acute Tox. 1 Inhalation - Acute Tox. 3 Oral - Acute Tox. 4 Dermal
• Storage Class: 6.1B - Non-combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• wgk_germany: WGK 3