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SRE0021

Sigma-Aldrich

Granzyme B human

recombinant, expressed in insect cells, ≥90% (SDS-PAGE)

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Synonym(s):
Granzyme B human, Cytotoxic T-lymphocyte proteinase 2, Granzyme-2, GranzymeB, Lymphocyte protease, SECT, T-cell serine protease 1-3E

biological source

human

Quality Level

recombinant

expressed in insect cells

assay

≥90% (SDS-PAGE)

form

lyophilized powder

potency

>0.004 units/mg

specific activity

≥40 units/μg

packaging

pkg of 5 μg

UniProt accession no.

shipped in

wet ice

storage temp.

−70°C

Gene Information

human ... GZMB(3002)

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SRP3202WH0003004M3SAB1303132
Sigma-Aldrich

SRE0021

Granzyme B human

Sigma-Aldrich

SRP3202

Granzyme B from mouse

biological source

human

biological source

mouse

biological source

mouse

biological source

rabbit

Gene Information

human ... GZMB(3002)

Gene Information

mouse ... GZMB(14939)

Gene Information

human ... GZMM(3004)

Gene Information

human ... GZMM(3004)

assay

≥90% (SDS-PAGE)

assay

≥97% (HPLC), ≥97% (SDS-PAGE)

assay

-

assay

-

recombinant

expressed in insect cells

recombinant

expressed in Hi-5 Insect cells

recombinant

-

recombinant

-

form

lyophilized powder

form

lyophilized

form

buffered aqueous solution

form

buffered aqueous solution

General description

Granzyme B (GzmB) is a serine protease which is stored in granules of activated cytotoxic T cells and NK cells. GZMB codes for granzyme B.

Biochem/physiol Actions

Granzyme B is considered as the marker of activated CTLs (cytotoxic T-cells), which mediates target-cell apoptosis. It induces apoptosis in caspase-dependent and caspase-independent manner. After contact with target cells, granzyme B is directionally exocytosed, and then enters the target cell with assistance from perforin. Granzyme B specifically cleaves after Asp residues, and particularly processes and activates various pro-caspases. This induces apoptosis in the target cell.

Unit Definition

One unit is defined as the amount of enzyme that hydrolyzes 1 pmol/min of IETD-AFC per min at 37°C in 50 mM HEPES, pH 7.5 containing 10 mM CaCl2.

Physical form

Lyophilized powder, containing HEPES buffer (original solution at pH 7.5)

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Granzyme B enters the mitochondria in a Sam50-, Tim22-and mtHsp70-dependent manner to induce apoptosis
Chiusolo V, et al.
Cell Death and Differentiation, 24(4), 747-747 (2017)
Risk of generalized vitiligo is associated with the common 55R-94A-247H variant haplotype of GZMB (encoding granzyme B).
Ferrara TM, et al.
The Journal of Investigative Dermatology, 133(6), 1677-1677 (2013)

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