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Cathepsin L Active human

recombinant, expressed in FreeStyle 293-F cells, ≥90% (SDS-PAGE)

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CATL, Major excreted protein (MEP)


expressed in FreeStyle 293-F cells


≥90% (SDS-PAGE)


aqueous solution

mol wt

36 kDa

NCBI accession no.

shipped in

dry ice

storage temp.


Gene Information

human ... CTSL(1514)

General description

Human cathepsin L (GenBank Accession No. NM_001912), amino acids 18-333, with C-terminal HIS tag, MW = 36 kDa, expressed in FreeStyle 293-F cells.


Active human cathepsin L is useful for the activation of DPP1 (Cathepsin C). Active human cathepsin L has been used in a study to determine that N-acyl and N-sulfonyloxazolidine-2,4-diones are pseudo-irreversible inhibitors of serine proteases. Active human cathepsin L has also been used in a study to investigate the role of cathepsin B and L activity in the serum during the human aging process.

Unit Definition

One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37°C

Physical form

Formulated in 50 mM MES, 400 mM NaCl, pH 5.0, and 10% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Legal Information

FreeStyle is a trademark of Invitrogen Corp.

Storage Class

10 - Combustible liquids




Not applicable


Not applicable

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