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SRP3261

Sigma-Aldrich

FGF-4 human

Animal-component free, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

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Synonym(s):
HBGF-4, HST-1, transforming protein KS3

biological source

human

recombinant

expressed in E. coli

assay

≥95% (HPLC)
≥95% (SDS-PAGE)

form

lyophilized

potency

≥0.5 ng/mL

mol wt

19.7 kDa

packaging

pkg of 25 μg

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

Storage temp.

−20°C

Gene Information

human ... FGF4(2249)

General description

The FGF4 (fibroblast growth factor 4) gene is mapped to human chromosome 11q13.3. It is expressed during early embryonic development. In adults, it is strongly expressed in the testis. The encoded protein mainly interacts with FGFR1c (FGF receptor 1c) and FGFR2c. It also binds to FGFR3c and FGFR4, but to a lesser extent.

Application

  • FGF-4 human has been used to study the effect of FGF4 on the differentiation of PDMCs (placenta-derived multipotent cells) into hepatocyte-like cells.
  • It has been used as a growth factor to study the proliferation and differentiation of human bone marrow mesenchymal stem cells (hMSCs) to chondrocytes.
  • It has been used to study hepatic differentiation.

Biochem/physiol Actions

FGF4 (fibroblast growth factor 4) was first identified as an oncogene transforming NIH3T3 (mouse embryo fibroblast cell line) cells. It helps in maintaining the self-renewal capability of normal stem cells (for instance, trophoblastic stem cells). It is also required for cellular differentiation, morphogenesis and proliferation. It controls proliferation and differentiation of normal stem cells, progenitor cells and cancer cells.

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.6 + 100 mM NaCl.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Increased FGF3 and FGF4 gene dosage is a risk factor for craniosynostosis.
Grillo L, et al.
Gene, 534, 435-435 (2014)
Isolation and characterization of epithelial progenitor cells from human fetal liver.
Liu YN, et al.
Hepatology Research : the Official Journal of the Japan Society of Hepatology, 38, 103-103 (2008)
Fibroblasts induce expression of FGF4 in ovarian cancer stem-like cells/cancer-initiating cells and upregulate their tumor initiation capacity.
Yasuda K, et al.
Laboratory Investigation; a Journal of Technical Methods and Pathology, 94, 1355-1355 (2014)
Chondrogenic induction of human mesenchymal stem cells using combined growth factors for cartilage tissue engineering.
Bosetti M, et al.
Journal of Tissue Engineering and Regenerative Medicine, 6, 205-205 (2012)
Eryn Wicklow et al.
PLoS genetics, 10(10), e1004618-e1004618 (2014-10-24)
Pluripotent epiblast (EPI) cells, present in the inner cell mass (ICM) of the mouse blastocyst, are progenitors of both embryonic stem (ES) cells and the fetus. Discovering how pluripotency genes regulate cell fate decisions in the blastocyst provides a valuable

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