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F8424

Sigma-Aldrich

Fibroblast Growth Factor-4 human

FGF-4, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

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Synonym(s):
hFGF-4, FGF-4, K-FGF
MDL number:
NACRES:
NA.77

biological source

human

Quality Level

recombinant

expressed in E. coli

assay

≥95% (SDS-PAGE and HPLC)

form

lyophilized powder

potency

≤10.0 ng/mL

quality

endotoxin tested

mol wt

19 kDa

packaging

pkg of 25 and 100 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

<1 EU/μg

UniProt accession no.

storage temp.

−20°C

Gene Information

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This Item
F5542F3685F7301
Sigma-Aldrich

F8424

Fibroblast Growth Factor-4 human

Fibroblast Growth Factor-Basic FGF-Basic, from human, recombinant, expressed in E. coli, carrier free

F3685

Fibroblast Growth Factor-Basic

Sigma-Aldrich

F7301

Fibroblast Growth Factor-18 human

assay

≥95% (SDS-PAGE and HPLC)

assay

≥97% (SDS-PAGE)

assay

≥97% (SDS-PAGE)

assay

>95% (SDS-PAGE and HPLC)

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

biological source

human

biological source

human

biological source

human

biological source

human

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

General description

Fibroblast Growth Factor-4 (FGF4) is a 19 kDa protein, belonging to the FGF family of proteins. Fibroblast growth factors (FGF) are glycoproteins concealed in the extracellular matrix and cell surface. There are around 22 different types of FGFs known. These factors weigh in the range of 17 to 34 kDa and they possess a conserved sequence of 120 amino acids. The FGF4 gene is mapped to human chromosome 11q13.3.

application

Fibroblast Growth Factor-4 human has been used:
  • To maintain trophectoderm stem cells.
  • In neurogenic differentiation.
  • In hepatocyte differentiation.

Biochem/physiol Actions

Fibroblast growth factor-4 (FGF-4) is a 19 kDa protein identified in NIH-3T3 cell assays as a transforming gene from DNA derived from human tumors, including stomach (hst) and colon cancers, Kaposi′s sarcoma, and hepatocellular carcinoma. FGF-4 includes a secretory signal sequence and shares 42% sequence identity with FGF-Basic at the amino acid level. Both FGF-4 and FGF-Basic bind to the same receptors. Mouse and human FGF-4 shares 82% homology with species cross-reactivity. FGF-4 does not appear to be expressed in normal adult tissues, but during embryogenesis, the gene is spatially and temporally regulated. FGF-4 is a mitogen for fibroblasts and endothelial cells and a potent promoter of angiogenesis. It is believed to be critical in embryonic limb development.
Fibroblast growth factor-4 (FGF-4) is identified in NIH-3T3 cell assays as a transforming gene from DNA derived from human tumors, including stomach (hst) and colon cancers, Kaposi′s sarcoma, and hepatocellular carcinoma. FGF-4 includes a secretory signal sequence and shares 42% sequence identity with FGF-Basic at the amino acid level. Both FGF-4 and FGF-Basic bind to the same receptors. Mouse and human FGF-4 shares 82% homology with species cross-reactivity. FGF-4 does not appear to be expressed in normal adult tissues, but during embryogenesis, the gene is spatially and temporally regulated. FGF-4 is a mitogen for fibroblasts and endothelial cells and a potent promoter of angiogenesis. It is believed to be critical in embryonic limb development. FGF-4 participates in cell differentiation and cell migration.

Physical form

Lyophilized from a 0.2 μm filtered buffer solution containing a carrier protein.

Analysis Note

The biological activity is measured by the dose-dependent stimulation of thymidine uptake using BaF3 cells expressing FGF receptors.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Riley McMahon et al.
Frontiers in cell and developmental biology, 9, 777652-777652 (2022-02-19)
The specification of anterior head tissue in the late gastrulation mouse embryo relies on signaling cues from the visceral endoderm and anterior mesendoderm (AME). Genetic loss-of-function studies have pinpointed a critical requirement of LIM homeobox 1 (LHX1) transcription factor in
Derivation, maintenance, and characterization of rat embryonic stem cells in vitro
Embryonic Stem Cell Protocols, 45-58 (2006)
The oncoprotein HBXIP up-regulates FGF4 through activating transcriptional factor Sp1 to promote the migration of breast cancer cells
Shi H, et al.
Biochemical and biophysical research communications, 471(1), 89-94 (2016)
Endometrial regenerative cells: a novel stem cell population
Meng X, et al.
Journal of Translational Medicine, 5(1), 57-57 (2007)
Fibroblast growth factors (FGFs).
Vainikka, S., et al. et al.
Guidebook to Cytokines and Their Receptors, 214-214 (1994)

Articles

Fibroblast growth factors in cell culture and various growth factors for your research

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