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Key Documents

SRP8050

Sigma-Aldrich

Profilin from Toxoplasma gondii

recombinant, expressed in E. coli, >95% (SDS-PAGE)

Synonym(s):

Inflammatory profilin, T Cell Membrane Protein 1

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

Toxoplasma gondii

recombinant

expressed in E. coli

assay

>95% (SDS-PAGE)

form

lyophilized

mol wt

~20 kDa by SDS-PAGE

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

<0.01 EU/μg endotoxin, tested

color

white

UniProt accession no.

Q58NA1

shipped in

wet ice

storage temp.

−20°C

Gene Information

Toxoplasma gondii ... PRF(7900190)

Biochem/physiol Actions

Profilin is a protozoan parasite protein. It activates toll-like receptor 11 (TLR11), which is an intracellular receptor localized on the endoplasmic reticulum (ER). TLR11 acts as an innate immune sensor for T. gondii profilin (TgPRF).The 12 membrane-spanning ER-resident protein UNC93B1 directly binds TLR11 and control the activation of dendritic cells in response to profilin (Toxoplasma gondii) and parasitic infection in vivo. TLR12 also has an ability to interact with (TgPRF). TLR12 is adequate for recognizing profilin in plasmacytoid dendritic cells (pDCs), whereas TLR11 and TLR12 are both needed in macrophages and conventional DCs. In contrast to TLR11, TLR12-deficient mice fail to resist T. gondii infection.

Physical form

Lyophilized from PBS.

Reconstitution

Reconstitute with 100 μL deionized water.

Other Notes

Recombinant profilin (Toxoplasma gondii) (aa 1-163).

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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UNC93B1 is essential for TLR11 activation and IL-12 dependent host resistance to Toxoplasma Gondii.
Pifer R, et al.
The Journal of Biological Chemistry, M110-M110 (2010)
Recognition of profilin by Toll-like receptor 12 is critical for host resistance to Toxoplasma gondii.
Koblansky A A, et al.
Immunity, 38(1), 119-130 (2013)
Erik W Martin et al.
Science signaling, 13(620) (2020-02-27)
The complex signaling dynamics of transcription factors can encode both qualitative and quantitative information about the extracellular environment, which increases the information transfer capacity and potentially supports accurate cellular decision-making. An important question is how these signaling dynamics patterns are

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