MilliporeSigma
All Photos(1)

Documents

T0813

Sigma-Aldrich

Anti-Tumor Necrosis Factor-α antibody produced in goat

affinity isolated antibody, lyophilized powder

Sign Into View Organizational & Contract Pricing

Synonym(s):
Anti-TNF-α
NACRES:
NA.41

biological source

goat

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

human

technique(s)

immunohistochemistry: suitable
neutralization: suitable
western blot: suitable

UniProt accession no.

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TNF(7124)

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
T1816T6817T8300
biological source

goat

biological source

goat

biological source

mouse

biological source

rabbit

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

200

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

IgG fraction of antiserum

Gene Information

human ... TNF(7124)

Gene Information

pig ... TNF(397086)

Gene Information

human ... TNF(7124)

Gene Information

human ... TNF(7124)

species reactivity

human

species reactivity

pig

species reactivity

human

species reactivity

human

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Tumor Necrosis Factor-α (TNF-α) is a cytokine produced by macrophages in response to stimulus by LPS. It plays a major role in mediating inflammation, tissue injury, pathogenic shock, innate immunity, apoptosis and autoimmunity. The physiological effects of TNF-α are mediated by receptors of TNFR super family. The TNF/TNFR signaling axis plays a critical role in mediating either survival or apoptosis depending on the class of adaptor proteins recruited in the cell. Association of receptors with a death domain causes rapid activation of caspases and results in cell death. TNF/TNFR also results in the activation of downstream pathways involving MAPK, p38, JNK and NF-κB that play a major role in innate immunity, acute inflammatory responses and homeostasis. The TNF/TNFR axis is also important in the pathology of various autoimmune and inflammatory disorders including a variety of malignancies and Alzheimer′s disease. Anti-Tumor Necrosis Factor-α antibody is specific for human TNF-α. The antibody shows less than 10% cross reactivity with recombinant rabbit TNF-α and less than 5% cross reactivity with recombinant mouse TNF-α.

Specificity

The antibody neutralizes the biological activity of recombinant human TNF-α. The antibody shows <10% cross-reactivity with recombinant rat TNF-α and <5% cross-reactivity with recombinant mouse TNF-α.

Immunogen

recombinant human TNF-α.

Application

Anti-Tumor Necrosis Factor-α (TNF- α) antibody produced in goat has been used to study the release of TNF- α from the cocultured lung carcinoma cells A427:AML-193.
Anti-Tumor Necrosis Factor-α antibody may be used for immunoblotting at a working antibody concentration of 0.1 μg/ml. For immunohistochemistry a concentration range of 5-15 μg/ml is recommended. The antibody may be used for neutralization assay; the ND50 is 0.01-0.06 μg/mL in the presence of 1.5ng/mL Recombinant Human TNF-alpha.

Physical form

Lyophilized from 0.2 μm filtered solution in phosphate buffered saline containing 5% trehalose.

Preparation Note

The antibody is purified using TNF-α affinity chromatography.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Biochanin A extirpates the epithelial-mesenchymal transition in a human lung cancer.
Wang Y, et al.
Experimental and Therapeutic Medicine, 15(3), 2830-2836 (2018)
The TNF receptor superfamily of cellular and viral proteins: activation, costimulation, and death.
C A Smith et al.
Cell, 76(6), 959-962 (1994-03-25)
K A Papadakis et al.
Annual review of medicine, 51, 289-298 (2000-04-25)
Recent advances in the drug treatment of inflammatory bowel disease (IBD) have paralleled our understanding of the pathophysiology of ulcerative colitis and Crohn's disease. Several proinflammatory and immune-regulatory cytokines are upregulated in the mucosa of patients with IBD, and differences
A Ashkenazi et al.
Science (New York, N.Y.), 281(5381), 1305-1308 (1998-08-28)
Apoptosis is a cell suicide mechanism that enables metazoans to control cell number in tissues and to eliminate individual cells that threaten the animal's survival. Certain cells have unique sensors, termed death receptors, on their surface. Death receptors detect the
Yan Wang et al.
Experimental and therapeutic medicine, 15(3), 2830-2836 (2018-02-20)
The natural iso-flavonoid, biochanin A, is categorized as a phytoestrogen and has been demonstrated to exhibit various pharmacological properties. However, no effects of biochanin A on lung cancer cell lines have been reported. Therefore, the present study aimed to demonstrate

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service