T5516

3,3′,5-Triiodo-L-thyronine sodium salt

γ-irradiated, powder, suitable for, suitable for cell culture

• Synonym(s): Liothyronine, O-(4-Hydroxy-3-iodophenyl)-3,5-diiodo-L-tyrosine sodium salt, T₃
• Empirical Formula (Hill Notation): C₁₅H₁₁I₃NNaO₄
• CAS Number: 55-06-1
• Molecular Weight: 672.96
• Beilstein: 8179867
• EC Number: 200-223-5
• MDL number: MFCD00002594
• PubChem Substance ID: 24900290
• NACRES: NA.77

Properties

• biological source: synthetic (organic)
• Quality Level: 200
• sterility: γ-irradiated
• Assay: ≥90% (HPLC)
• form: powder
• technique(s): cell culture | mammalian: suitable
• suitability: suitable for
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: [Na+].N[C@@H](Cc1cc(I)c(Oc2ccc(O)c(I)c2)c(I)c1)C([O-])=O
• InChI: 1S/C15H12I3NO4.Na/c16-9-6-8(1-2-13(9)20)23-14-10(17)3-7(4-11(14)18)5-12(19)15(21)22;/h1-4,6,12,20H,5,19H2,(H,21,22);/q;+1/p-1/t12-;/m0./s1
• InChI key: SBXXSUDPJJJJLC-YDALLXLXSA-M
• Gene Information: human ... THRA(7067) , THRB(7068) ; rat ... Thra(81812) , Thrb(24831)

Description

General description

3,3′,5′-Triiodo-L-thyronine (T3) is an endocrine hormone produced by the thyroid gland and is regarded as an active thyroid hormone at cellular level. Thyroxine (T4) serves as a precursor for T3 and is generated in extrathyroidal tissues.

Application

3,3′,5-Triiodo-L-thyronine sodium salt has been used:

• as a supplement in colon organ culture media
• to stimulate HuH-7 cells for analysing the hormonal influence on MBL synthesis
• to study its effect on human growth hormone (GH) versus mouse GH expression in the presence of ′′intact′′ GH genes and in a common pituitary cell context

Biochem/physiol Actions

3,3′,5′-Triiodo-L-thyronine (T3) is a thyroid hormone that increases rates of proteins synthesis, stimulates the breakdown of cholesterol, and effects embryonic development. In cell culture, T3 regulates cell differentiation and protein expression. The interaction of T3 with nonhistone proteins in the chromatin initiates the thyroid hormone activity. Thyroid hormones regulate the differentiation and development of tissues and affect a number of metabolic processes. The level of circulating T3 hormone is reduced in hypothyroidism.

Physical form

powder-0 °C; stock-frozen in working aliquots, avoid repeated freeze/thaw, solution stable 2-8 °C, 30 days

Preparation Note

Package size based on 3,3′,5-Triiodo-L-thyronine sodium salt content; vial may also contain inert salts, such as sodium chloride

Reconstitution

To prepare 20 μg/ml stock solution: add 1 ml 1 N NaOH; gently swirl to dissolve; add 49 ml sterile medium.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves