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T6763

Sigma-Aldrich

Trypsin Acetylated from bovine pancreas

Type V-S, ≥8,500 BAEE units/mg protein (biuret)

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Synonym(s):
Acetyltrypsin
Enzyme Commission number:
MDL number:
NACRES:
NA.54

biological source

bovine pancreas

Quality Level

type

Type V-S

form

powder

specific activity

≥8,500 BAEE units/mg protein (biuret)

UniProt accession no.

storage temp.

−20°C

Gene Information

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This Item
T8802T1005T1143
Trypsin from bovine pancreas TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein

T8802

Trypsin from bovine pancreas

Trypsin from bovine pancreas Type XI, lyophilized powder, ≥6,000 BAEE units/mg protein

T1005

Trypsin from bovine pancreas

Sigma-Aldrich

T1143

Trypsinogen from bovine pancreas

specific activity

≥8,500 BAEE units/mg protein (biuret)

specific activity

≥10,000 BAEE units/mg protein

specific activity

≥6,000 BAEE units/mg protein

specific activity

≥10,000 BAEE units/mg protein (E1%/280, after activation to trypsin)

Gene Information

cow ... TRYP8(282603)

Gene Information

-

Gene Information

-

Gene Information

bovine ... TRYP8(282603)

form

powder

form

essentially salt-free, lyophilized powder

form

lyophilized powder

form

essentially salt-free, lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

UniProt accession no.

Q29463

UniProt accession no.

-

UniProt accession no.

-

UniProt accession no.

Q29463

General description

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

Application

Trypsin has been used in a study to assess the ontogeny of the endocrine pancreas in the fetal/newborn baboon. Trypsin digestion and hydrogen/deuterium exchange support the transition between inward- and outward-facing conformations during the catalytic cycle of the bacterial multidrug ATP-binding cassette transporter.

Biochem/physiol Actions

Lys-acetylated trypsin is less susceptible to autolysis than the native form.

Unit Definition

One BAEE unit will produce a ΔA253 of 0.001 per min at pH 7.6 at 25° C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).

Preparation Note

Prepared by the method of Labouesse, J. and Gervais, M., Eur. J. Biochem., 2, 215 (1967).

Other Notes

View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer.

pictograms

Exclamation markHealth hazard

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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