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T9455

Sigma-Aldrich

TMB Enhanced One Component HRP Membrane Substrate

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Synonym(s):
HRP substrate, TMB, detection reagent, detection substrate
NACRES:
NA.83

form

solution

Quality Level

200

storage temp.

2-8°C

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ES022ES001T0565
Sigma-Aldrich

Sigma-Aldrich

T9455

TMB Enhanced One Component HRP Membrane Substrate

Millipore

Millipore

ES022

TMB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate (soluble)

Millipore

Millipore

ES001

TMB/E Single Reagent, Blue color, Horseradish Peroxidase Substrate (soluble)

3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes ready to use solution

Sigma-Aldrich

T0565

3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes

storage temp.

2-8°C

storage temp.

-

storage temp.

-

storage temp.

2-8°C

Quality Level

200

Quality Level

100

Quality Level

-

Quality Level

100

General description

TMB Enhanced One Component HRP Membrane Substrate is a ready-to-use solution containing 3,3′,5,5′-tetramethylbenzidine (TMB) in a mildly acidic buffer. TMB is a substrate for horseradish peroxidase. The substrate system will react with sites on the membrane containing peroxidase, producing an insoluble permanent dark blue reaction product. To stop the reaction, rinse the membrane with reagent quality water. This substrate is not recommended for microwell or immunohistochemical applications.

Application

TMB Enhanced One Component HRP Membrane Substrate has been used as a colorimetric substrate for horseradish peroxidase (HRP) in an electrochemical enzymatic sandwich assay for the detection of interleukin-6 (IL-6) in biological fluids.

Caution

Stable at least one year at 2-8 °C.

Physical form

Ready-to-use.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Graphene Enabled Low-Noise Surface Chemistry for Multiplexed Sepsis Biomarker Detection in Whole Blood
Zupancic U, et al.
Advances in Functional Materials, 31(16), 2010638-2010638 (2021)

Protocols

Introduction to Western Blot Detection of Proteins

Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

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