ZMS1156
Anti-β Actin Antibody, clone 4C2 ZooMAb® Mouse Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Actin cytoplasmic 1
About This Item
Recommended Products
biological source
mouse
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
4C2, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 41.74 kDa
observed mol wt ~40 kDa
purified by
using protein G
species reactivity
monkey, mouse, human, rat
packaging
antibody small pack of 25 μL
greener alternative product characteristics
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enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1κ
epitope sequence
N-terminal
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
Gene Information
human ... ACTB(60)
General description
Specificity
Immunogen
Application
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected β Actin in HeLa cell lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected β Actin in Jurkat, COS-7, NIH3T3, and PC12 cell lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected β Actin in human small intestine and human epididymis tissue sections.
Flow Cytometry Analysis: 1 µg from a representative lot detected β Actin in one million HeLa cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Physical form
Reconstitution
Storage and Stability
Other Notes
Legal Information
Disclaimer
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
Follow this short antibody reconstitution protocol and see how easy it is to reconstitute ZooMAb® recombinant antibodies.
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