ZRB1761
Anti-ZO-1 Antibody, clone 4E14 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
About This Item
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biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
4E14, monoclonal
recombinant monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt ~195.46 kDa
observed mol wt ~250 kDa
species reactivity
human
species reactivity (predicted by homology)
monkey
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
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Design for Energy Efficiency
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enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG
UniProt accession no.
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Specificity
Immunogen
Application
Evaluated by Western Blotting in A549 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ZO-1 in A549 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ZO-1 in HepG2, MCF-7, and NIH3T3 cell lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ZO-1 in human kidney tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ZO-1 in MCF-7 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Physical form
Reconstitution
Storage and Stability
Legal Information
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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