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567225-U

Supelco

Discovery® BIO Wide Pore C8 HPLC Column

10 μm particle size, L × I.D. 25 cm × 21.2 mm

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eCl@ss:
32110501
NACRES:
SB.52

material

stainless steel column

Quality Level

agency

suitable for USP L7

product line

Discovery®

feature

endcapped

manufacturer/tradename

Discovery®

packaging

1 ea of

extent of labeling

5% Carbon loading

parameter

0-70 °C temperature
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

25 cm × 21.2 mm

surface area

100 m2/g

surface coverage

4 μmol/m2

matrix

silica particle platform
fully porous particle

matrix active group

C8 (octyl) phase

particle size

10 μm

pore size

300 Å

operating pH

2-8

separation technique

reversed phase

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General description

Discovery BIO Wide Pore C8 exhibits hydrophobicity intermediate between the Discovery BIO Wide Pore C5 and the Discovery BIO Wide Pore C18. The difference in hydrophobicity gives it unique selectivity relative to these other phases. It is ideal for peptide mapping because it provides complementary information compared to a C18 separation. Because of its intermediate hydrophobicity, we also recommend it for method development or scouting work. As with all Discovery BIO Wide Pore phases, the C8 phase gives efficient, symmetrical peaks, exceptional stability, long column lifetime, and LC-MS compatibility.

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Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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