Documents
66825-U
BIOshell™ A400 Protein C4, 3.4 μm HPLC Column
3.4 μm particle size, L × I.D. 10 cm × 2.1 mm
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material
stainless steel hardware
Quality Level
Agency
suitable for USP L26
description
Shell thickness (0.2 μm)
Solid Core (3.0 μm)
product line
BIOshell™
feature
endcapped
manufacturer/tradename
BIOshell™
packaging
1 ea of
extent of labeling
0.4% carbon loading
parameter
600 bar max. pressure
90 °C max. temp.
technique(s)
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
L × I.D.
10 cm × 2.1 mm
surface area
15 m2/g
surface coverage
4.2 μmol/m2
matrix
spherical silica particle platform
superficially porous particle
matrix active group
C4 (butyl) phase
particle size
3.4 μm
pore size
400 Å
operating pH range
2-9
separation technique
reversed phase
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Related Categories
1 of 4
This Item | 66828-U | 66824-U | 66826-U |
|---|---|---|---|
| agency suitable for USP L26 | agency suitable for USP L26 | agency suitable for USP L26 | agency suitable for USP L26 |
| product line BIOshell™ | product line BIOshell™ | product line BIOshell™ | product line BIOshell™ |
| feature endcapped | feature endcapped | feature endcapped | feature endcapped |
| packaging 1 ea of | packaging 1 ea of | packaging 1 ea of | packaging 1 ea of |
| extent of labeling 0.4% carbon loading | extent of labeling 0.4% carbon loading | extent of labeling 0.4% carbon loading | extent of labeling 0.4% carbon loading |
General description
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Articles
Optimization of a Reversed-Phase Liquid Chromatographic (RP-LC) method for the intact analysis of a therapeutic monoclonal antibody, trastuzumab, using a BIOshell™ A400 Protein C4 column.
The article describes the use of difluoroacetic acid (DFA) as an effective alternative to acid modifiers, formic acid (FA) and trifluoroacetic acid (TFA) in the LC-UV/MS analysis of proteins.
-casein basis (electrophoresis), lyophilized powder; β-Casein from bovine milk, BioUltra, ≥98% (PAGE)
BIOshell™ A400 Protein C4 columns contain 3.4 μm particles with 400 Å pores, which are derivatized with dimethylbutyl silane and exhaustively endcapped for optimum protein recovery.
Protocols
Larger porous shell particles with narrow particle size distribution, used in Supelco's BIOshell™ columns for the reversed-phase U/HPLC analysis of peptides and proteins, provide increased efficiency per unit pressure drop.
BIOshell™ IgG 1000 Å C4 UHPLC Column for Improved Biomacromolecule Separations
HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell™ A400 Protein C4
Related Content
High performance liquid chromatography (HPLC) can be used to separate and identify different large biomolecules such as protein and peptides in a sample. It is based on the pumping of a sample with a solvent (mobile phase) through a column packed with sorbent material (stationary phase) at a high pressure.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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