Skip to Content
MilliporeSigma
  • Characterization, cloning, and heterologous expression of a subtilisin-like serine protease gene VlPr1 from Verticillium lecanii.

Characterization, cloning, and heterologous expression of a subtilisin-like serine protease gene VlPr1 from Verticillium lecanii.

Journal of microbiology (Seoul, Korea) (2013-01-01)
Gang Yu, Jin-Liang Liu, Li-Qin Xie, Xue-Liang Wang, Shi-Hong Zhang, Hong-Yu Pan
ABSTRACT

The entomopathogenic fungus Verticillium lecanii is a well-known biocontrol agent. V. lecanii produces subtilisin-like serine protease (Pr1), which is important in the biological control activity of some insect pests by degrading insect cuticles. In this study, a subtilisin-like serine protease gene VlPr1 was cloned from the fungus and the VlPr1 protein was expressed in Escherichia coli. The VlPr1 gene contains an open reading frame (ORF) interrupted by three short introns, and encodes a protein of 379 amino acids. Protein sequence analysis revealed high homology with subtilisin serine proteases. The molecular mass of the protease was 38 kDa, and the serine protease exhibited its maximal activity at 40°C and pH 9.0. Protease activity was also affected by Mg(2+) and Ca(2+) concentration. The protease showed inhibitory activity against several plant pathogens, especially towards Fusarium moniliforme.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Proteinase, bacterial, Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 g
Estimated to ship on March 22, 2025
$131.00
1 kg
Estimated to ship on March 22, 2025
$328.00
50 kg
Estimated to ship on March 22, 2025
$12,800.00
Sigma-Aldrich
Protease from Bacillus licheniformis, Type VIII, lyophilized powder, 7-15 units/mg solid
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 g
Estimated to ship on March 22, 2025
$131.00
1 kg
Estimated to ship on March 22, 2025
$328.00
50 kg
Estimated to ship on March 22, 2025
$12,800.00
Sigma-Aldrich
Protease from Bacillus licheniformis, lyophilized powder, for use in Total Dietary Fiber Assay, TDF-100A
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 g
Estimated to ship on March 22, 2025
$131.00
1 kg
Estimated to ship on March 22, 2025
$328.00
50 kg
Estimated to ship on March 22, 2025
$12,800.00
Sigma-Aldrich
Protease from Bacillus licheniformis, glycerol solution (50%)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 g
Estimated to ship on March 22, 2025
$131.00
1 kg
Estimated to ship on March 22, 2025
$328.00
50 kg
Estimated to ship on March 22, 2025
$12,800.00
Sigma-Aldrich
Protease from Bacillus licheniformis, ≥2.4 U/g
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 g
Estimated to ship on March 22, 2025
$131.00
1 kg
Estimated to ship on March 22, 2025
$328.00
50 kg
Estimated to ship on March 22, 2025
$12,800.00