Skip to Content
MilliporeSigma
  • Thrombolytic effects of Douchi fibrinolytic enzyme from Bacillus subtilis LD-8547 in vitro and in vivo.

Thrombolytic effects of Douchi fibrinolytic enzyme from Bacillus subtilis LD-8547 in vitro and in vivo.

BMC biotechnology (2012-07-04)
Jun Yuan, Jing Yang, Zhenhong Zhuang, Yanling Yang, Ling Lin, Shihua Wang
ABSTRACT

Today, thrombosis is one of the most widely occurring diseases in modern life. Drugs with thrombolytic functions are the most effective methods in the treatment of thrombosis. Among them, Douchi fibrinolytic enzyme (DFE) is a promising agent. DFE was isolated from Douchi, a typical and popular soybean-fermented food in China, and it can dissolve fibrin directly and efficiently. A strain, Bacillus subtilis LD-8547 produced DFE with high fibrinolytic activity has been isolated in our lab previously. In the study, thrombolytic effect of DFE from Bacillus subtilis LD-8547 was studied in vitro and in vivo systematically. The results showed that DFE played a significant role in thrombolysis and anticoagulation in vitro. And the thrombolytic effects correlated with DFE in a dose-dependent manner. In vivo, the acute toxicity assay showed that DFE had no obvious acute toxicity to mice. Test of carrageenan-induced thrombosis in mice indicated that the DFE significantly prevented tail thrombosis, and arterial thrombosis model test indicated that Douchi fibrinolytic enzyme DFE had thrombolytic effect on carotid thrombosis of rabbits in vivo. Other results in vivo indicated that DFE could increase bleeding and clotting time obviously. The DFE isolated from Bacillus subtilis LD-8547 has obvious thrombolytic effects in vitro and in vivo. This function demonstrates that this enzyme can be a useful tool for preventing and treating clinical thrombus.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Proteinase, bacterial, Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$260.00
$182.00
Sigma-Aldrich
Protease from Bacillus licheniformis, lyophilized powder, for use in Total Dietary Fiber Assay, TDF-100A
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$260.00
$182.00
Sigma-Aldrich
Protease from Bacillus licheniformis, Type VIII, lyophilized powder, 7-15 units/mg solid
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$260.00
$182.00
Sigma-Aldrich
Protease from Bacillus licheniformis, ≥2.4 U/g
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$260.00
$182.00
Sigma-Aldrich
Protease from Bacillus licheniformis, glycerol solution (50%)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 ea
In Stock
Details...
$260.00
$182.00