Skip to Content
MilliporeSigma
  • Effect of phenol-induced changes in lipid composition on conformation of OmpF-like porin of Yersinia pseudotuberculosis.

Effect of phenol-induced changes in lipid composition on conformation of OmpF-like porin of Yersinia pseudotuberculosis.

FEBS letters (2013-06-08)
Nina Sanina, Sanina Nina, Ludmila Davydova, Davydova Ludmila, Svetlana Bakholdina, Bakholdina Svetlana, Olga Novikova, Novikova Olga, Olga Pornyagina, Pornyagina Olga, Tamara Solov'eva, Solov'eva Tamara, Valery Shnyrov, Shnyrov Valery, Mikhail Bogdanov, Bogdanov Mikhail
ABSTRACT

The present work aimed to compare the effects of different lysophosphatidylethanolamine (LPE) content in lipids derived from Yersinia pseudotuberculosis cells exposed and not exposed to phenol on the conformation of OmpF-like porin of these bacteria. Differential scanning calorimetry and intrinsic protein fluorescence showed that the 2.5-fold increase of LPE content and the corresponding increase in the phase transition temperature of bacterial lipids were accompanied by enhanced protein thermostability. Integral conformational rearrangement of protein was supported by drastic changes in the microenvironment of the tryptophan residues, likely resulting in a convergence of monomers in trimeric porin and exposure of outer tryptophan residues to the water environment. These conformational changes may impede the porin channel permeability under stress conditions in bacteria.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Pandy′s reagent
Sigma-Aldrich
Phenol stock solution, 100 mg/dL, standard
Sigma-Aldrich
Phenol, JIS special grade, ≥99.0%
Sigma-Aldrich
Phenol, SAJ first grade, ≥98.0%
Sigma-Aldrich
Phenol, ≥99.0%
Sigma-Aldrich
Phenol, ≥99.0%
Supelco
Phenol solution, 5000 μg/mL in methanol, certified reference material
Sigma-Aldrich
Phenol, unstabilized, purified by redistillation, ≥99%
Sigma-Aldrich
Phenol, unstabilized, ReagentPlus®, ≥99%
Supelco
Phenol solution, certified reference material, 500 μg/mL in methanol
Supelco
Phenol, PESTANAL®, analytical standard
Sigma-Aldrich
Phenol, puriss., meets analytical specification of Ph. Eur., BP, USP, 99.5-100.5% (GC)
Sigma-Aldrich
Phenol, puriss., meets analytical specification of Ph. Eur., BP, USP, ≥99.5% (GC), crystalline (detached)
Sigma-Aldrich
Phenol, ≥96.0% (calc. on dry substance, T)
Sigma-Aldrich
Phenol, ≥99%
Sigma-Aldrich
Phenol, contains hypophosphorous as stabilizer, loose crystals, ACS reagent, ≥99.0%
Supelco
Phenol solution, 100 μg/mL in acetonitrile, PESTANAL®, analytical standard
Sigma-Aldrich
Phenol, puriss. p.a., ACS reagent, reag. Ph. Eur., 99.0-100.5%
Sigma-Aldrich
Phenol, BioUltra, for molecular biology, TE-saturated, ~73% (T)
Sigma-Aldrich
Phenol, BioUltra, for molecular biology, ≥99.5% (GC)
Sigma-Aldrich
Phenol solution, Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, for molecular biology
Sigma-Aldrich
Phenol, BioXtra, ≥99.5% (GC)
Sigma-Aldrich
Liquified Phenol, ≥89.0%
Sigma-Aldrich
Phenol solution, BioReagent, Saturated with 0.01 M citrate buffer, pH 4.3 ± 0.2, for molecular biology
Sigma-Aldrich
Phenol, for molecular biology
Supelco
Phenol, Pharmaceutical Secondary Standard; Certified Reference Material