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  • Fabrication and characterization of size-controlled starch-based nanoparticles as hydrophobic drug carriers.

Fabrication and characterization of size-controlled starch-based nanoparticles as hydrophobic drug carriers.

Journal of nanoscience and nanotechnology (2013-11-20)
Fei Han, Chunmei Gao, Mingzhu Liu
ABSTRACT

Acetylated corn starch was successfully synthesized and optimized by the reaction of native corn starch with acetic anhydride and acetic acid in the presence of sulfuric acid as a catalyst. The optimal degree of substitution of 2.85 was obtained. Starch-based nanoparticles were fabricated by a simple and novel nanoprecipitation procedure, by the dropwise addition of water to acetone solution of acetylated starch under stirring. Fourier transform infrared spectrometry showed that acetylated starch had some new bands at 1750, 1375 and 1240 cm(-1) while acetylated starch nanoparticles presented the identical peaks as the drug-loaded acetylated starch nanoparticles and the acetylated starch. Wide angle X-ray diffraction indicated that A-type pattern of native starch was completely transformed into the V-type pattern of Acetylated starch and starch-based nanoparticles show the similar type pattern with the acetylated starch. The scanning electron microscopy showed that the different sizes of pores formed on the acetylated starch granules were utterly converted into the uniform-sized spherical nanoparticles after the nanoprecipitation. The encapsulation efficiency and diameter of nanoparticle can be adjusted by the degree of substitution, the volume ratio of nonsolvent to solvent and the weight ratio of acetylated starch to drug. It was also depicted that the release behaviors of drug-loaded nanoparticles depend on the size of nanoparticles and the degree of substitution of the acetylated starch. Release studies prove that the starch-based nanoparticles with uniform size can be used for the encapsulation of hydrophobic drug and attained the sustained and controllable drug release carriers.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Starch, puriss. p.a., from potato, reag. ISO, reag. Ph. Eur., soluble
Supelco
Starch from corn, for use with Total Dietary Fiber Control Kit, TDF-C10
Supelco
Starch from corn, analytical standard, analytical standard for Starch Assay Kits SA-20 and STA-20
Sigma-Aldrich
Starch from corn
Sigma-Aldrich
Starch from potato, Powder
Sigma-Aldrich
Starch from corn, practical grade
Sigma-Aldrich
Starch from corn, Unmodified waxy corn starch of essentially pure amylopectin; contains only trace amounts of amylose.
Sigma-Aldrich
Starch from rice
Sigma-Aldrich
Starch from wheat, Unmodified
Sigma-Aldrich
Starch from potato, suitable for electrophoresis
Sigma-Aldrich
Starch from potato, Soluble
Sigma-Aldrich
Starch, from potato, tested according to Ph. Eur.