Skip to Content
MilliporeSigma
  • Protease inhibitor from insect silk-activities of derivatives expressed in vitro and in transgenic potato.

Protease inhibitor from insect silk-activities of derivatives expressed in vitro and in transgenic potato.

Applied biochemistry and biotechnology (2013-07-05)
Dalibor Kodrík, Barbara Kludkiewicz, Oldřich Navrátil, Oxana Skoková Habuštová, Vendulka Horáčková, Zdeňka Svobodová, Konstantin S Vinokurov, František Sehnal
ABSTRACT

Several recombinant derivatives of serine protease inhibitor called silk protease inhibitor 2 (SPI2), which is a silk component in Galleria mellonella (Lepidoptera, Insecta), were prepared in the expression vector Pichia pastoris. Both the native and the recombinant protease inhibitors were highly active against subtilisin and proteinase K. The synthetic SPI2 gene with Ala codon in the P1 position was fused with mGFP-5 to facilitate detection of the transgene and its protein product. A construct of the fusion gene with plant regulatory elements (promoter 35S and terminator OCS) was inserted into the binary vector pRD400. The final construct was introduced into Agrobacterium tumefaciens that was then used for genetic transformation of the potato variety Velox. The transgene expression was monitored with the aid of ELISA employing polyclonal antibody against natural SPI2. In vitro tests showed increased resistance to the late blight Phytophthora infestans in several transformed lines. No effect was seen on the growth, mortality, life span or reproduction of Spodoptera littoralis (Lepidoptera, Insecta) caterpillars, while feeding on transformed potato plants expressing the fusion protein, indicating that the transformed potatoes may be harmless to non-target organisms.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Proteinase, bacterial, Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
15000000 units
Estimated to ship on March 22, 2025
$200.00
75000000 units
Estimated to ship on March 22, 2025
$687.00
150000000 units
Estimated to ship on March 22, 2025
$1,170.00
Sigma-Aldrich
Protease from Bacillus licheniformis, Type VIII, lyophilized powder, 7-15 units/mg solid
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
15000000 units
Estimated to ship on March 22, 2025
$200.00
75000000 units
Estimated to ship on March 22, 2025
$687.00
150000000 units
Estimated to ship on March 22, 2025
$1,170.00
Sigma-Aldrich
Protease from Bacillus licheniformis, lyophilized powder, for use in Total Dietary Fiber Assay, TDF-100A
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
15000000 units
Estimated to ship on March 22, 2025
$200.00
75000000 units
Estimated to ship on March 22, 2025
$687.00
150000000 units
Estimated to ship on March 22, 2025
$1,170.00
Sigma-Aldrich
Protease from Bacillus licheniformis, glycerol solution (50%)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
15000000 units
Estimated to ship on March 22, 2025
$200.00
75000000 units
Estimated to ship on March 22, 2025
$687.00
150000000 units
Estimated to ship on March 22, 2025
$1,170.00
Sigma-Aldrich
Protease from Bacillus licheniformis, ≥2.4 U/g
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
15000000 units
Estimated to ship on March 22, 2025
$200.00
75000000 units
Estimated to ship on March 22, 2025
$687.00
150000000 units
Estimated to ship on March 22, 2025
$1,170.00