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Molecular cytogenetics of human single pronucleated zygotes.

Molecular cytogenetics of human single pronucleated zygotes.

Reproductive sciences (Thousand Oaks, Calif.) (2014-04-11)

Ana Raquel Azevedo, Maria João Pinho, Joaquina Silva, Rosália Sá, Sólveig Thorsteinsdóttir, Alberto Barros, Mário Sousa

ABSTRACT

The aim of the present study was to use fluorescence in situ hybridization to analyze the chromosome status of zygotes with a single pronucleus from in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) treatment cycles. In addition, we performed immunocytochemical detection of nuclear lamins and histone H3 trimethylated at lysine-9, Me(3)H3K9. Zygotes were processed 24 hours after insemination or injection to assure the absence of asynchrony. In opposition to previous results, we observed 2 pronuclei in 16 of 18 IVF zygotes and 40 of 64 ICSI zygotes, suggesting premature pronuclear breakdown. In IVF and ICSI zygotes, the rate of normal diploidy was only 6 of 16 and 27 of 56, respectively, suggesting that monopronucleated zygotes should not be used in assisted reproductive treatments. The possible mechanisms are discussed and compared to previous studies of monopronucleated zygotes.

MATERIALS

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