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Bioaccessibility of selenium, selenomethionine and selenocysteine from foods and influence of heat processing on the same.

Bioaccessibility of selenium, selenomethionine and selenocysteine from foods and influence of heat processing on the same.

Food chemistry (2015-10-17)

Anjum Khanam, Kalpana Platel

ABSTRACT

Selenium (Se) is an essential nutrient with diverse physiological functions. The selenium content of commonly consumed cereals, pulses and green leafy vegetables (GLV) was determined. Bioaccessibility of Se, and its organic forms selenomethionine (SeMet), and selenocysteine (SeCys2) was also examined, and the effect of heat processing on the same was studied. The bioaccessibility of Se in cereals ranged from 10% to 24%, that of pulses was between 12% and 29%, and of GLV, 10-31%. The concentration of SeMet in the dialysates of the cereals, pulses and GLV ranged from 5.15 to 28.7, 2.7 to 36.2, and 0.03 to 5ngg(-1), respectively. The concentration of SeCys2 in the dialysates of the foods examined was negligible. Heat processing significantly decreased the bioaccessibility of Se, SeMet and SeCys2. This is the first report on the bioaccessibility of Se and its major organic forms from commonly consumed staples, and the effect of heat processing on the same.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Germanium(IV) oxide, (crystalline powder), 99.998% trace metals basis

Sigma-Aldrich

Methanol, anhydrous, 99.8%

Sigma-Aldrich

Selenium, powder, −100 mesh, 99.99% trace metals basis

Sigma-Aldrich

Selenium, pellets, <5 mm particle size, ≥99.999% trace metals basis

Sigma-Aldrich

Methanol, suitable for HPLC, gradient grade, 99.93%

Sigma-Aldrich

Germanium(IV) oxide, powder, 99.999% trace metals basis

Sigma-Aldrich

Selenium, pellets, <5 mm, ≥99.99% trace metals basis

Sigma-Aldrich

Selenium, powder, −100 mesh, ≥99.5% trace metals basis

Sigma-Aldrich

Germanium(IV) oxide, ≥99.99% trace metals basis

Sigma-Aldrich

Methanol, NMR reference standard

Sigma-Aldrich

Methanol, HPLC Plus, ≥99.9%, poly-coated bottles

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Methanol, SAJ special grade

Sigma-Aldrich

Methanol, suitable for HPLC

Sigma-Aldrich

Methanol, JIS special grade, ≥99.8%

Sigma-Aldrich

Methanol, SAJ first grade, ≥99.5%

Sigma-Aldrich

Methanol, JIS 300, ≥99.8%, for residue analysis

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

Sigma-Aldrich

Methanol, Absolute - Acetone free

Sigma-Aldrich

Methanol, BioReagent, ≥99.93%

Sigma-Aldrich

Methanol, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8% (GC)

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

Sigma-Aldrich

Methanol, puriss., meets analytical specification of Ph Eur, ≥99.7% (GC)

Sigma-Aldrich

Methanol, Laboratory Reagent, ≥99.6%

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

Sigma-Aldrich

Methanol, ACS spectrophotometric grade, ≥99.9%

Selenium, pellets, < 5mm, ≥99.999%

Selenium, foil, 25x25mm, thickness 3mm, 99.95%

Sigma-Aldrich

Formic acid, ACS reagent, ≥88%

Sigma-Aldrich

Formic acid, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥98%

Sigma-Aldrich

Formic acid, puriss., meets analytical specifications of DAC, FCC, 98.0-100%