Skip to Content
MilliporeSigma
  • Characterization of a novel cometabolic degradation carbazole pathway by a phenol-cultivated Arthrobacter sp. W1.

Characterization of a novel cometabolic degradation carbazole pathway by a phenol-cultivated Arthrobacter sp. W1.

Bioresource technology (2015-07-06)
Shengnan Shi, Yuanyuan Qu, Hao Zhou, Qiao Ma, Fang Ma
ABSTRACT

Arthrobacter sp. W1 was used to characterize the pathways involved in cometabolic degradation of carbazole (CA) with phenol as the primary substrate. To clarify the upper pathway of cometabolic degradation CA, Escherichia coli strain BL21 expressing phenol hydroxylase from strain W1 (PHIND) was investigated to degrade CA. Firstly, CA was initially monohydroxylated at C-2 and C-4 positions to produce 2- and 4-hydroxycarbazole, followed by successively hydroxylated to the corresponding 1,2- and 3,4-dihydroxycarbazole, of which 3,4-dihydroxycarbazole was unequivocally identified for the first time. To characterize the downstream cometabolic degradation CA pathway, purified 3,4-dihydroxycarbazole was used as the substrate for phenol-grown W1, and a series of novel indole derivatives were identified. These results suggested that a novel pathway of CA catabolism was employed by strain W1 via a successive hydroxylation and meta-cleavage pathway. These findings provide new insights into the cometabolic degradation CA process and have potential applications in biotechnology and bioremediation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Pyrocatechol, ≥99%
Sigma-Aldrich
Pyrocatechol, purified by sublimation, ≥99.5%
Sigma-Aldrich
Isatin, 97%
Sigma-Aldrich
Liquified Phenol, ≥89.0%
Sigma-Aldrich
Phenol, BioXtra, ≥99.5% (GC)
Sigma-Aldrich
Phenol, puriss., ≥99.5% (GC), meets analytical specification of Ph. Eur., BP, USP, crystalline (detached)
Sigma-Aldrich
Phenol, puriss., meets analytical specification of Ph. Eur., BP, USP, 99.5-100.5% (GC)
Sigma-Aldrich
Phenol, ≥96.0% (calc. on dry substance, T)
Sigma-Aldrich
Phenol, puriss. p.a., ACS reagent, reag. Ph. Eur., 99.0-100.5%
Sigma-Aldrich
Phenol, contains hypophosphorous as stabilizer, loose crystals, ACS reagent, ≥99.0%
Sigma-Aldrich
Phenol, unstabilized, ReagentPlus®, ≥99%
Sigma-Aldrich
Phenol, unstabilized, purified by redistillation, ≥99%
Sigma-Aldrich
4-Hydroxycarbazole, 95%
Sigma-Aldrich
1,2-Dihydroxybenzene, ReagentPlus®, ≥99%
Sigma-Aldrich
Phenol, ≥99.0%
Sigma-Aldrich
Phenol, ≥99.0%
Sigma-Aldrich
Phenol, SAJ first grade, ≥98.0%
Sigma-Aldrich
Phenol, JIS special grade, ≥99.0%
Sigma-Aldrich
Phenol, natural, 97%, FG
Sigma-Aldrich
3-Indoleacrylic acid, powder
Sigma-Aldrich
3-Indoleacrylic acid, BioReagent, ≥98% (HPLC)
Sigma-Aldrich
Phenol, BioUltra, for molecular biology, TE-saturated, ~73% (T)
Sigma-Aldrich
Phenol, for molecular biology
Sigma-Aldrich
Phenol solution, BioReagent, Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, for molecular biology
Sigma-Aldrich
Phenol, ≥99%
Sigma-Aldrich
Phenol solution, Saturated with 0.01 M citrate buffer, pH 4.3 ± 0.2, BioReagent, for molecular biology