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  • Photocrosslinkable hyaluronan-gelatin hydrogels for two-step bioprinting.

Photocrosslinkable hyaluronan-gelatin hydrogels for two-step bioprinting.

Tissue engineering. Part A (2010-04-15)
Aleksander Skardal, Jianxing Zhang, Lindsi McCoard, Xiaoyu Xu, Siam Oottamasathien, Glenn D Prestwich
ABSTRACT

Bioprinting by the codeposition of cells and biomaterials is constrained by the availability of printable materials. Herein we describe a novel macromonomer, a new two-step photocrosslinking strategy, and the use of a simple rapid prototyping system to print a proof-of-concept tubular construct. First, we synthesized the methacrylated ethanolamide derivative of gelatin (GE-MA). Second, partial photochemical cocrosslinking of GE-MA with methacrylated hyaluronic acid (HA-MA) gave an extrudable gel-like fluid. Third, the new HA-MA:GE-MA hydrogels were biocompatible, supporting cell attachment and proliferation of HepG2 C3A, Int-407, and NIH 3T3 cells in vitro. Moreover, hydrogels injected subcutaneously in nude mice produced no inflammatory response. Fourth, using the Fab@Home printing system, we printed a tubular tissue construct. The partially crosslinked hydrogels were extruded from a syringe into a designed base layer, and irradiated again to create a firmer structure. The computer-driven protocol was iterated to complete a cellularized tubular construct with a cell-free core and a cell-free structural halo. Cells encapsulated within this printed construct were viable in culture, and gradually remodeled the synthetic extracellular matrix environment to a naturally secreted extracellular matrix. This two-step photocrosslinkable biomaterial addresses an unmet need for printable hydrogels useful in tissue engineering.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Gelatin methacryloyl, gel strength 300 g Bloom, degree of substitution 40%
Sigma-Aldrich
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Sigma-Aldrich
Gelatin methacryloyl, gel strength 300 g Bloom, 80% degree of substitution
Sigma-Aldrich
Gelatin methacryloyl, gel strength 300 g Bloom, degree of substitution 60%
Sigma-Aldrich
TissueFab® bioink , (Gel)ma -UV/365 nm
Sigma-Aldrich
Gelatin methacryloyl, gel strength 170-195 g Bloom, degree of substitution: 60%
Sigma-Aldrich
Low endotoxin GelMA, mol wt 95 kDa, degree of substitution 60%
Sigma-Aldrich
Low endotoxin GelMA, gel strength 300 (Bloom), degree of substitution 80%, GelMA Type B
Sigma-Aldrich
Low endotoxin GelMA, bloom 300, Type A, degree of substitution 80%
Sigma-Aldrich
TissueFab® bioink , Alg(Gel)ma -UV/365 nm
Sigma-Aldrich
TissueFab® bioink , (GelAlgHA)MA Vis/405 nm, low endotoxin
Sigma-Aldrich
TissueFab® bioink , (Gel)ma -VIS/405nm, low endotoxin
Sigma-Aldrich
Gelatin methacryloyl, gel strength 90-110 g Bloom, degree of substitution 60%
Sigma-Aldrich
TissueFab® - low endotoxin GelMA-UV bioink, 0.2 μm filtered, suitable for 3D bioprinting applications
Sigma-Aldrich
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Sigma-Aldrich
Hyaluronic acid methacrylate, Degree of substitution: 10% - 30%, Mw 170,000-250,000
Sigma-Aldrich
Hyaluronic acid methacrylate, degree of substitution 20-50%, Mw 40-70 kDa
Sigma-Aldrich
Hyaluronic acid methacrylate, degree of substitution 20-50%, Mw 140-190 kDa
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TissueFab® bioink kit, Fibronectin-UV/365nm
Sigma-Aldrich
TissueFab® bioink kit, (Gel)ma Fibrin (Vis/405), low endotoxin
Sigma-Aldrich
Low endotoxin GelMA solution, gel strength 300 (bloom), degree of substitution 80%, 0.2 μm, sterile-filtered, GelMA Type B
Sigma-Aldrich
TissueFab® GelAlg − LAP Bioink, low endotoxin, 0.2 μm filtered, suitable for 3D bioprinting applications
Sigma-Aldrich
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Sigma-Aldrich
TissueFab® bioink kit, (Gel)ma Fibrin (UV/365), low endotoxin
Sigma-Aldrich
TissueFab® bioink kit, (Gel)ma Laminin -UV/365 nm, low endotoxin
TissueFab® Discrete GelMA, 300 bloom, 80% degree of substitution
Sigma-Aldrich
TissueFab® bioink kit, (Gel)ma Laminin -Vis/405 nm, low endotoxin