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HPLC Analysis of Leucine Enantiomers on Astec® CHIROBIOTIC® T (25 °C)

HPLC Analysis of Leucine Enantiomers on Astec® CHIROBIOTIC® T (25 °C) application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Astec® CHIROBIOTIC® T Chiral HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

guard cartridge

Product No.
Description
Pricing

Astec® CHIROBIOTIC® T Chiral HPLC Guard Column

Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm

standard

Product No.
Description
Pricing

DL-Leucine

≥99% (HPLC)

L-Leucine

from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%

L-Leucine

reagent grade, ≥98% (HPLC)

L-Leucine

tested according to Ph. Eur.

L-Leucine

BioUltra, ≥99.5% (NT)

DL-β-Leucine

≥98.0% (NT)

D-Leucine

ReagentPlus®, 99%

L-Leucine

99%, FG

L-Leucine

certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

CONDITIONS

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] water; [B] methanol; [C] formic acid; (30:70:0.02, A:B:C)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 205 nm

injection

10 μL

sample

300 μg /mL in 30:70 water:methanol

Description

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC