MilliporeSigma

HPLC Analysis of Synthetic Food Dyes on Ascentis® Express C18

HPLC Analysis of Synthetic Food Dyes on Ascentis® Express C18 application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Ascentis® Express C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

standard

Product No.
Description
Pricing

Phloxine B

Dye content ≥80 %, certified by the Biological Stain Commission

Rose bengal

certified by the Biological Stain Commission, Dye content ≥80 %

Erioglaucine disodium salt

coloring dye

Tartrazine

analytical standard

Sunset Yellow FCF

analytical standard

Allura Red AC

analytical standard

Amaranth

analytical standard

Erythrosin B

analytical standard

Fast Green FCF

analytical standard

Indigo carmine

analytical standard

CONDITIONS

column

Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)

mobile phase

[A] 100 mM ammonium phosphate monobasic, pH 7.0 with ammonium hydroxide; [B] water; [C] acetonitrile

gradient

10% A constant; 85 to 25% B, 5 to 65% C in 6 min; 25% B, 65% C held for 5 min

flow rate

0.3 mL/min

column temp.

40 °C

detector

UV, 254 nm

injection

5 μL

sample

20 μg/mL each in water

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany