Skip to Content
Merck
HomeMammalian Cell CultureMinimum Essential Medium Eagle (MEM) Formulation

Minimum Essential Medium Eagle (MEM) Formulation

Minimum Essential Medium Eagle (MEM)

ComponentM0268
g/L
M0275
[10x] g/L
M0446
[1x] g/L
M0643
g/L
M1018
g/L
Inorganic Salts
Calcium Chloride0.220.20.20.1396
Magnesium Sulfate (anhydrous)0.097670.97670.097670.097670.09767
Potassium Chloride0.440.40.40.4
Potassium Phosphate Monobasic (anhydrous)0.06
Sodium Bicarbonate2.2
Sodium Chloride6.8686.86.88
Sodium Phosphate Dibasic (anhydrous)0.04788
Sodium Phosphate Monobasic (anhydrous)0.1221.220.1220.122
Amino Acids
L-Alanine0.00890.0089
L-Alanyl-L-Glutamine0.4344
L-Arginine • HCl0.1261.260.1260.1260.126
L-Asparagine • H2O0.0150.015
L-Aspartic Acid0.01330.0133
L-Cystine • 2HCl0.03130.3130.03130.03130.0313
L-Glutamic Acid0.01470.0147
L-Glutamine0.2920.2920.2920.292
Glycine0.00750.0075
L-Histidine • HCl • H2O0.0420.420.0420.0420.042
L-Isoleucine0.0520.520.0520.0520.052
L-Leucine0.0520.520.0520.0520.052
L-Lysine • HCl0.07250.7250.07250.07250.0725
L-Methionine0.0150.150.0150.0150.015
L-Phenylalanine0.0320.320.0320.0320.032
L-Proline0.01150.0115
L-Serine0.01050.0105
L-Threonine0.0480.480.0480.0480.048
L-Tryptophan0.010.10.010.010.01
L-Tyrosine • 2Na • 2H2O0.05190.5190.05190.05190.0519
L-Valine0.0460.460.0460.0460.046
Vitamins
Choline Chloride0.0010.010.0010.0010.001
Folic Acid0.0010.010.0010.0010.001
myo-Inositol0.0020.020.0020.0020.002
Niacinamide0.0010.010.0010.0010.001
D-Pantothenic Acid (hemicalcium)0.0010.010.0010.0010.001
Pyridoxal • HCl0.0010.010.0010.0010.001
Riboflavin0.00010.0010.00010.00010.0001
Thiamine • HCl0.0010.010.0010.0010.001
Other
Glucose110111
Phenol Red • Na0.0110.110.0110.0110.011
Add
L-Glutamine0.292 at 1×
Sodium Bicarbonate2.22.2 at 1×2.20.35

Minimum Essential Medium Eagle (MEM) (cont.)

ComponentM2279
g/L
M2414
[1x] g/L
M4642
[1x] g/L
M4655
g/L
Inorganic Salts
Calcium Chloride0.20.20.20.2
Magnesium Sulfate (anhydrous)0.097670.097670.097670.09767
Potassium Chloride0.40.40.40.4
Potassium Phosphate Monobasic (anhydrous)0.06
Sodium Bicarbonate2.22.22.2
Sodium Chloride6.86.886.8
Sodium Phosphate Dibasic (anhydrous)0.04788
Sodium Phosphate Monobasic (anhydrous)0.1220.1220.122
Amino Acids
L-Alanine
L-Arginine • HCl0.1260.1260.1260.126
L-Asparagine • H2O
L-Aspartic Acid
L-Cystine • 2HCl0.03130.03130.03130.0313
L-Glutamic Acid
L-Glutamine0.2920.2920.292
Glycine
L-Histidine • HCl • H2O0.0420.0420.0420.042
L-Isoleucine0.0520.0520.0520.052
L-Leucine0.0520.0520.0520.052
L-Lysine • HCl0.07250.07250.07250.0725
L-Methionine0.0150.0150.0150.015
L-Phenylalanine0.0320.0320.0320.032
L-Proline
L-Serine
L-Threonine0.0480.0480.0480.048
L-Tryptophan0.010.010.010.01
L-Tyrosine • 2Na • 2H2O0.05190.05190.05190.0519
L-Valine0.0460.0460.0460.046
Vitamins
Choline Chloride0.0010.0010.0010.001
Folic Acid0.0010.0010.0010.001
myo-Inositol0.0020.0020.0020.002
Niacinamide0.0010.0010.0010.001
D-Pantothenic Acid (hemicalcium)0.0010.0010.0010.001
Pyridoxal • HCl0.0010.0010.0010.001
Riboflavin0.00010.00010.00010.0001
Thiamine • HCl0.0010.0010.0010.001
Other
Glucose1111
Phenol Red • Na0.0110.0110.0110.011
Add
L-Glutamine0.292
Sodium Bicarbonate0.35

Minimum Essential Medium Eagle (MEM) (cont.)

ComponentM4780
[1x] g/L
M5650
[1x] g/L
M5775
[1x] g/L
M9288
[10x] g/L
Inorganic Salts
Calcium Chloride0.13960.20.13961.396
Magnesium Sulfate (anhydrous)0.097670.097670.097670.9767
Potassium Chloride0.40.40.44
Potassium Phosphate Monobasic (anhydrous)0.060.060.6
Sodium Bicarbonate0.352.20.35
Sodium Chloride86.8880
Sodium Phosphate Dibasic (anhydrous)0.047880.047880.4788
Sodium Phosphate Monobasic (anhydrous)0.122
Amino Acids
L-Alanine0.0089
L-Arginine • HCl0.1260.1260.1261.26
L-Asparagine • H2O0.015
L-Aspartic Acid0.0133
L-Cystine • 2HCl0.03130.03130.03130.313
L-Glutamic Acid0.0147
L-Glutamine0.292
Glycine0.0075
L-Histidine • HCl • H2O0.0420.0420.0420.42
L-Isoleucine0.0520.0520.0520.52
L-Leucine0.0520.0520.0520.52
L-Lysine • HCl0.07250.07250.07250.725
L-Methionine0.0150.0150.0150.15
L-Phenylalanine0.0320.0320.0320.32
L-Proline0.0115
L-Serine0.0105
L-Threonine0.0480.0480.0480.48
L-Tryptophan0.010.010.010.1
L-Tyrosine • 2Na • 2H2O0.05190.05190.05190.519
L-Valine0.0460.0460.0460.46
Vitamins
Choline Chloride0.0010.0010.0010.01
Folic Acid0.0010.0010.0010.01
myo-Inositol0.0020.0020.0020.02
Niacinamide0.0010.0010.0010.01
D-Pantothenic Acid (hemicalcium)0.0010.0010.0010.01
Pyridoxal • HCl0.0010.0010.0010.01
Riboflavin0.00010.00010.00010.001
Thiamine • HCl0.0010.0010.0010.01
Other
Glucose11110
Phenol Red • Na0.0110.0110.0110.11
Add
L-Glutamine0.2920.2920.292 at 1×
Sodium Bicarbonate0.35 at 1×

Minimum Essential Medium Eagle (MEM) Alpha Modification

ComponentM0644
g/L
M0894
g/L
M4526
[1x] g/L
M8042
[1x] g/L
Inorganic Salts
Calcium Chloride0.20.20.20.2
Magnesium Sulfate (anhydrous)0.097670.097670.097670.09767
Potassium Chloride0.40.40.40.4
Sodium Bicarbonate2.22.2
Sodium Chloride6.86.86.86.8
Sodium Phosphate Monobasic (anhydrous)0.1220.1220.1220.122
Amino Acids
L-Alanine0.0250.0250.0250.025
L-Arginine • HCl0.1260.1260.1260.126
L-Asparagine • H2O0.050.050.050.05
L-Aspartic Acid0.030.030.030.03
L-Cysteine • HCl • H2O0.10.10.10.1
L-Cystine • 2HCl0.03130.03130.03130.0313
L-Glutamic Acid0.0750.0750.0750.075
L-Glutamine0.2920.292
Glycine0.050.050.050.05
L-Histidine • HCl • H2O0.0420.0420.0420.042
L-Isoleucine0.0520.0520.0520.052
L-Leucine0.0520.0520.0520.052
L-Lysine • HCl0.07250.07250.07250.0725
L-Methionine0.0150.0150.0150.015
L-Phenylalanine0.0320.0320.0320.032
L-Proline0.040.040.040.04
L-Serine0.0250.0250.0250.025
L-Threonine0.0480.0480.0480.048
L-Tryptophan0.010.010.010.01
L-Tyrosine • 2Na • 2H2O0.05190.05190.05190.0519
L-Valine0.0460.0460.0460.046
Vitamins
L-Ascorbic Acid • Na0.050.050.050.05
Biotin0.00010.00010.00010.0001
Choline Chloride0.0010.0010.0010.001
Folic Acid0.0010.0010.0010.001
myo-Inositol0.0020.0020.0020.002
Lipoic Acid0.0002
Niacinamide0.0010.0010.0010.001
D-Pantothenic Acid (hemicalcium)0.0010.0010.0010.001
Pyridoxal • HCl0.0010.0010.0010.001
Riboflavin0.00010.00010.00010.0001
Thiamine • HCl0.0010.0010.0010.001
Vitamin B120.001360.001360.001360.00136
Other
Adenosine0.010.01
Cytidine0.010.01
2'-Deoxyadenosine0.010.01
2'-Deoxycytidine • HCl0.0110.011
2'-Deoxyguanosine0.010.01
Glucose1111
Guanosine0.010.01
Phenol Red • Na0.0110.0110.0110.011
Pyruvic Acid • Na0.110.110.110.11
Thioctic Acid0.00020.00020.0002
Thymidine0.010.01
Uridine0.010.01
Add
L-Glutamine0.2920.292
Sodium Bicarbonate2.22.2

Minimum Essential Medium Eagle (MEM) HEPES Modification

ComponentM2645
g/L
M7278
[1x] g/L
Inorganic Salts
Calcium Chloride0.20.2
Magnesium Sulfate (anhydrous)0.097670.09767
Potassium Chloride0.40.4
Sodium Bicarbonate2.2
Sodium Chloride5.55.5
Sodium Phosphate Monobasic (anhydrous)0.1220.122
Amino Acids
L-Arginine • HCl0.1260.126
L-Cystine • 2HCl0.03130.0313
L-Glutamine0.292
L-Histidine • HCl • H2O0.0420.042
L-Isoleucine0.0520.052
L-Leucine0.0520.052
L-Lysine • HCl0.07250.0725
L-Methionine0.0150.015
L-Phenylalanine0.0320.032
L-Threonine0.0480.048
L-Tryptophan0.010.01
L-Tyrosine • 2Na • 2H2O0.05190.0519
L-Valine0.0460.046
Vitamins
Choline Chloride0.0010.001
Folic Acid0.0010.001
myo-Inositol0.0020.002
Niacinamide0.0010.001
D-Pantothenic Acid (hemicalcium)0.0010.001
Pyridoxal • HCl0.0010.001
Riboflavin0.00010.0001
Thiamine • HCl0.0010.001
Other
Glucose11
HEPES5.9585.958
Phenol Red • Na0.0110.011
Add
L-Glutamine0.292
Sodium Bicarbonate2.2

Minimum Essential Medium Eagle (MEM) Joklik Modification for Suspension Cultures

ComponentM0518
g/L
M8028
[1x] g/L
Inorganic Salts
Magnesium Chloride • 6H2O0.20.2
Potassium Chloride0.40.4
Sodium Bicarbonate2
Sodium Chloride6.56.5
Sodium Phosphate Monobasic (anhydrous)1.1541.154
Amino Acids
L-Arginine • HCl0.1261.26
L-Cystine • 2HCl0.03240.0324
L-Glutamine0.292
L-Histidine • HCl • H2O0.0420.042
L-Isoleucine0.0520.052
L-Leucine0.0520.052
L-Lysine • HCl0.07250.0725
L-Methionine0.0150.015
L-Phenylalanine0.0320.032
L-Threonine0.0480.048
L-Tryptophan0.010.01
L-Tyrosine • 2Na • 2H2O0.054520.05452
L-Valine0.0460.046
Vitamins
Choline Chloride0.0010.001
Folic Acid0.0010.001
myo-Inositol0.0020.002
Niacinamide0.0010.001
D-Pantothenic Acid (hemicalcium)0.0010.001
Pyridoxal • HCl0.0010.001
Riboflavin0.00010.0001
Thiamine • HCl0.0010.001
Other
Glucose22
Phenol Red • Na0.0110.011
Add
L-Glutamine0.292
Sodium Bicarbonate2

Minimum Essential Medium Eagle (MEM) Modified

ComponentM3024
g/L
Inorganic Salts
Calcium Chloride0.2
Magnesium Sulfate (anhydrous)0.09767
Potassium Chloride0.4
Sodium Chloride6.8
Sodium Phosphate Monobasic (anhydrous)0.122
Amino Acids
L-Alanine0.0089
L-Arginine • HCl0.126
L-Asparagine • H2O0.015
L-Aspartic Acid0.0133
L-Cystine • 2HCl0.0313
L-Glutamic Acid0.0147
Glycine0.0075
L-Histidine • HCl • H2O0.042
L-Isoleucine0.052
L-Leucine0.052
L-Lysine • HCl0.0725
L-Methionine0.015
L-Phenylalanine0.032
L-Proline0.0115
L-Serine0.0105
L-Threonine0.048
L-Tryptophan0.01
L-Tyrosine • 2Na • 2H2O0.0519
L-Valine0.046
Vitamins
Choline Chloride0.001
Folic Acid0.001
myo-Inositol0.002
Niacinamide0.001
D-Pantothenic Acid (hemicalcium)0.001
Pyridoxal • HCl0.001
Riboflavin0.0001
Thiamine • HCl0.001
Other
Glucose1
Add
L-Glutamine0.292
Sodium Bicarbonate2.2

Minimum Essential Medium Eagle (MEM) Auto-Mod™ Modified for Autoclaving

ComponentM0769
g/L
Inorganic Salts
Calcium Chloride0.2
Magnesium Sulfate (anhydrous)0.09767
Potassium Chloride0.4
Sodium Chloride6.8
Sodium Phosphate Monobasic (anhydrous)0.122
Sodium Succinate • 6H2O0.1
Succinic Acid (free acid)0.075
Amino Acids
L-Arginine • HCl0.126
L-Cystine • 2HCl0.0313
L-Histidine • HCl • H2O0.042
L-Isoleucine0.052
L-Leucine0.052
L-Lysine • HCl0.0725
L-Methionine0.015
L-Phenylalanine0.032
L-Threonine0.048
L-Tryptophan0.01
L-Tyrosine (free base)0.036
L-Valine0.046
Vitamins
Choline Bitartrate0.0018
Folic Acid0.001
myo-Inositol0.002
Niacinamide0.001
D-Pantothenic Acid (hemicalcium)0.001
Pyridoxal • HCl0.001
Riboflavin0.0001
Thiamine • HCl0.001
Other
Glucose1
Phenol Red • Na0.0064
Add
L-Glutamine0.292
Sodium Bicarbonate2.2

Minimum Essential Medium Eagle (S-MEM) Spinner Modification

ComponentM8167
[1x] g/L
Inorganic Salts
Magnesium Sulfate (anhydrous)0.09767
Potassium Chloride0.4
Sodium Chloride6.8
Sodium Phosphate Monobasic (anhydrous)0.122
Amino Acids
L-Arginine • HCl0.126
L-Cystine • 2HCl0.0313
L-Histidine • HCl • H2O0.042
L-Isoleucine0.052
L-Leucine0.052
L-Lysine • HCl0.0725
L-Methionine0.015
L-Phenylalanine0.032
L-Threonine0.048
L-Tryptophan0.01
L-Tyrosine • 2Na • 2H2O0.0519
L-Valine0.046
Vitamins
Choline Chloride0.001
Folic Acid0.001
myo-Inositol0.002
Niacinamide0.001
D-Pantothenic Acid (hemicalcium)0.001
Pyridoxal • HCl0.001
Riboflavin0.0001
Thiamine • HCl0.001
Other
Glucose1
Phenol Red • Na0.011
Sodium Bicarbonate2.2
Add
L-Glutamine0.292
Sign In To Continue

To continue reading please sign in or create an account.

Don't Have An Account?