L040150

Cap A

• 別名: Cap A
• UNSPSCコード: 12352106
• NACRES: NA.21

特性

• タイプ: for DNA synthesis
• 品質水準: 200
• 製品種目: Proligo Reagents
• フォーム: liquid
• 保管条件: dry at room temperature
• テクニック: DNA synthesis: suitable
• 不純物: ≤200 ppm water content (Karl Fischer)
• 色: clear
• 適合性: conforms to structure (GC, Formulation)
• 保管温度: room temp

詳細

詳細

Cap 1 solution consists of n-methylimidazole, lutidine, and acetonitrile (ACN).

アプリケーション

Cap 1 is suitable for use after the phosphoramidite reaction/coupling step.

生物化学的／生理学的作用

Cap 1 solution aborts chains bearing unreacted 5′hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.

調製ノート

Acetonitrile / Lutidine / N-Methylimidazole 8/1/1 (v/v/v)

安全性情報

• ピクトグラム: GHS02,GHS08,GHS05,GHS07
• シグナルワード: Danger
• 危険有害性情報: H225,H302 + H312 + H332,H314,H361d
• 注意書き: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• 危険有害性の分類: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Repr. 2 - Skin Corr. 1B
• 保管分類コード: 3 - Flammable liquids
• WGK: WGK 3
• 引火点(°F): 35.6 °F - closed cup
• 引火点(℃): 2.0 °C - closed cup